The fate of micronucleated cells post X-irradiation detected by live cell imaging

DNA Repair
Yun HuangQinghua Shi

Abstract

Micronuclei are closely related to DNA damage. The presence of micronuclei in mammalian cells is a common phenomenon post ionizing radiation. The level of micronucleation in tumor cells has been used to predict prognosis after radiotherapy in many cancers. In order to understand how irradiation-induced micronuclei affect cell fate, we performed extensive long-term live cell imaging on X-irradiated nasopharyngeal carcinoma (NPC) cells. To visualize the dynamics of micronuclei more clearly, chromosomes were stably labeled with red fluorescent protein (RFP) by targeting to human histone H2B. Initially, significantly more micronuclei were observed in radiosensitive cells than in radioresistant cells post irradiation. Additionally, cells with micronuclei were found to be more likely to die or undergo cell cycle arrest when compared with micronucleus-free cells after irradiation, and the more micronuclei the cells contained the more likely they would die or undergo arrest. Moreover, micronucleated cells showed predisposition to produce daughter cells with micronuclei through chromosome lagging. Fluorescence in situ hybridization using human pan-centromeric probes revealed that about 70% of these micronuclei and lagging chromosomes di...Continue Reading

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Citations

Feb 22, 2012·Cell Research·Yun HuangQinghua Shi
Jan 3, 2013·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Alka Bhatia, Yashwant Kumar
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Jan 1, 2013·The Journal of Contemporary Dental Practice·Syed SadatullahRafi Ahmad Togoo
Sep 14, 2021·The Journal of Cell Biology·Leslie A SepaniacJason Stumpff

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