The Fatty Acid β-Oxidation Pathway is Activated by Leucine Deprivation in HepG2 Cells: A Comparative Proteomics Study

Scientific Reports
Guokai YanXianghua Yan

Abstract

Leucine (Leu) is a multifunctional essential amino acid that plays crucial role in various cellular processes. However, the integral effect of Leu on the hepatic proteome remains largely unknown. Here, we for the first time applied an isobaric tags for relative and absolute quantification (iTRAQ)-based comparative proteomics strategy to investigate the proteome alteration induced by Leu deprivation in human hepatocellular carcinoma (HepG2) cells. A total of 4,111 proteins were quantified; 43 proteins were further identified as differentially expressed proteins between the normal and Leu deprivation groups. Bioinformatics analysis showed that the differentially expressed proteins were involved in various metabolic processes, including amino acid and lipid metabolism, as well as degradation of ethanol. Interestingly, several proteins involved in the fatty acid β-oxidation pathway, including ACSL1, ACADS, and ACOX1, were up-regulated by Leu deprivation. In addition, Leu deprivation led to the reduction of cellular triglycerides in HepG2 cells. These results reveal that the fatty acid β-oxidation pathway is activated by Leu deprivation in HepG2 cells, and provide new insights into the regulatory function of Leu in multiple cellular...Continue Reading

References

Feb 28, 1977·Biochimica Et Biophysica Acta·R Rognstad
May 1, 1973·The Biochemical Journal·J D McGivanJ B Chappell
Jun 11, 2002·Annual Review of Nutrition·Sidney M Morris
Aug 26, 2003·The Journal of Biological Chemistry·Angela M HallDavid A Bernlohr
Mar 17, 2004·Trends in Pharmacological Sciences·Etienne Quertemont, Sophie Tambour
Jul 9, 2004·Drug Metabolism Reviews·Vasilis VasiliouTia Estey
Apr 28, 2006·American Journal of Physiology. Endocrinology and Metabolism·Christopher J LynchThomas C Vary
Aug 24, 2006·JAMA : the Journal of the American Medical Association·Bianca T van MaldegemFrits A Wijburg
Nov 23, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Lin XinZheng-Gang Zhu
Jun 5, 2007·Molecular & Cellular Proteomics : MCP·Nicolas DenielSandrine Thébault
Mar 3, 2010·Journal of Inherited Metabolic Disease·Sander Michel Houten, Ronald J A Wanders
Oct 27, 2010·Expert Review of Proteomics·Achim Treumann, Bernd Thiede
Mar 23, 2011·Trends in Endocrinology and Metabolism : TEM·Hanqing Dong, Mark J Czaja
Jun 30, 2011·Human Genomics·Brian JacksonVasilis Vasiliou
Nov 6, 2012·Cell Metabolism·Clemence Blouet, Gary J Schwartz
Apr 16, 2013·Archives of Pharmacal Research·Kung-Woo NamMan-Deuk Han
Aug 14, 2013·The Journal of Biological Chemistry·Ainara G CabodevillaEnrique Claro
Dec 3, 2014·The International Journal of Biochemistry & Cell Biology·Longxiang XieJianping Xie
Jan 27, 2015·Molecular & Cellular Proteomics : MCP·Qi ShiXiao-Ping Dong
Apr 15, 2015·Biochimie·Guokai Yan, Xianghua Yan
Oct 27, 2015·International Journal of Obesity : Journal of the International Association for the Study of Obesity·H BhatiaM Datta

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