The fecal, oral, and skin microbiota of children with Chagas disease treated with benznidazole

PloS One
Carlos RobelloMaria G Dominguez-Bello

Abstract

Chagas disease is still prevalent in rural areas of South America. In endemic areas of Bolivia, school children are screened for the program of Chagas disease eradication of the Ministry of Health, and positive children are treated. Here, we compared the fecal, oral and skin microbiomes of children with or without Chagas disease, and before and after benznidazol treatment of infected children. A total of 543 Bolivian children (5-14 years old) were tested for Chagas disease, and 20 positive children were treated with Benznidazole. Fecal samples and oral and skin swabs were obtained before and after treatment, together with samples from a group of 35 uninfected controls. The 16S rRNA genes were sequenced and analyzed using QIIME to determine Alpha diversity differences and community distances, and linear discriminant analyses to determine marker taxa by infection status or treatment. Twenty out of 543 children screened were seropositive for Chagas disease (3.7%) and were included in the study, together with 35 control children that were seronegative for the disease. Fecal samples, oral and skin swabs were taken at the beginning of the study and after the anti-protozoa therapy with Benznidazole to the chagasic children. Infected c...Continue Reading

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Citations

Sep 26, 2020·Frontiers in Cellular and Infection Microbiology·Marcela de Souza-BasqueiraEster Cerdeira Sabino
Dec 15, 2020·British Journal of Clinical Pharmacology·Fernanda LascanoJaime Altcheh
Sep 29, 2020·PLoS Neglected Tropical Diseases·Nathalie de Sena PereiraPaulo Marcos Matta Guedes
Dec 4, 2020·Translational Psychiatry·Eduardo Duarte-SilvaChristina Alves Peixoto
Dec 29, 2020·Frontiers in Microbiology·Eduardo Duarte-SilvaChristina Peixoto

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Datasets Mentioned

BETA
ERP113722

Methods Mentioned

BETA
Assay

Software Mentioned

LEfSe
QIIME
RDP Classifier
Emperor
join
uclust
PyNAST
UniFrac
phyloSeq
fastq

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