The Ferritin-Heavy-Polypeptide-Like-17 (FTHL17) gene encodes a ferritin with low stability and no ferroxidase activity and with a partial nuclear localization

Biochimica Et Biophysica Acta
Paola RuzzenentiMaura Poli

Abstract

Three functional ferritin genes have been identified so far in mammals, and they encode the cytosolic Heavy (FTH) and Light chain (FTL) and the mitochondrial ferritin. The expression of a transcript by a fourth ferritin-like gene (Ferritin-Heavy-Polypeptide-Like-17, FTHL17) on the X chromosome was reported in mouse spermatogonia and in early embryonic cells. The intronless human FTHL17 gene encodes a protein with 64% identity to human FTH with substitution of key residues of the ferroxidase center. The gene was cloned into vectors for expression in Escherichia coli and mammalian cells, linked to a flag-tag. The recombinant FTHL17 from E. coli purified as an assembled 24-mer ferritin devoid of ferroxidase activity and with a reduced physical stability. When transiently expressed in mammalian cells the flag-FTHL17 assembled in ferritin shells that showed reduced stability to denaturants compared with flag H and L ferritins. Immunocytochemistry with anti-flag antibody decorated the nuclei of flag-FTHL17 transfected COS cells, but not those of the cells transfected with flag-FTH or flag-FTL. We concluded that FTHL17 encodes a ferritin-like protein without ferroxidase activity. Its restricted embryonic expression and partial nuclear...Continue Reading

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Citations

May 10, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Le LiMato Knez
Mar 16, 2016·Dose-response : a Publication of International Hormesis Society·Wei SunLiying Wang
Nov 1, 2015·The Biochemical Journal·Paolo ArosioMaura Poli
Dec 3, 2020·Cells·Maddalena Di SanzoMaria Concetta Faniello
Apr 16, 2021·Proceedings of the National Academy of Sciences of the United States of America·Pouya DiniBarry A Ball
Nov 28, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Magdalena GryzikMaura Poli

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