The Fes protein-tyrosine kinase phosphorylates a subset of macrophage proteins that are involved in cell adhesion and cell-cell signaling.

The Journal of Biological Chemistry
M JückerR A Feldman

Abstract

The c-fps/fes proto-oncogene encodes a 92-kDa protein-tyrosine kinase that is expressed at high levels in macrophages. We have previously shown that overexpression of c-fps/fes in a CSF-1-dependent macrophage cell line (BAC1.2F5) partially released these cells from their factor dependence and that this correlated with the tyrosine phosphorylation of a subset of proteins in a tissue-specific manner. We have now identified one of the macrophage substrates of Fes as the crk-associated substrate (Cas) and a second substrate as a 130-kDa protein that has been previously described as a T cell activation-dependent substrate and is unrelated to Cas. Both of these proteins, which have optimal consensus sequences for phosphorylation by Fes, were tightly associated with this kinase through its SH2 domain, suggesting that they were direct substrates of Fes. Remarkably, when the Fes SH2 domain was used as an affinity reagent to identify potential substrates of endogenous Fes in control BAC1.2F5 cells, the phosphotyrosyl proteins that were recognized were the same as those that were specifically phosphorylated when Fes was overexpressed in the same cells. We conclude that the substrates we identified may be structurally related or identical ...Continue Reading

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Citations

Sep 29, 2000·Biochemical Pharmacology·C CansG Superti-Furga
Mar 15, 2000·The International Journal of Biochemistry & Cell Biology·M Martins-GreenM Sudol
Mar 23, 2002·Molecular and Cellular Biology·Ralph A ZirngiblPeter A Greer
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Aug 18, 2009·Cellular Microbiology·Anna FahlgrenMaria Fällman
May 25, 2007·Journal of Cellular and Molecular Medicine·Shigeru KandaYasuyoshi Miyata
Sep 30, 1999·Microbial Pathogenesis·N HamidM Fällman
Mar 31, 2016·Biophysical Journal·Hengameh ShamsMohammad R K Mofrad
May 29, 2002·Oncogene·Blanca Scheijen, James D Griffin
May 8, 2002·Nature Reviews. Molecular Cell Biology·Peter Greer
Mar 14, 2002·International Journal of Medical Microbiology : IJMM·Maria FällmanKaren McGee

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