The fibroblast integrin alpha11beta1 is induced in a mechanosensitive manner involving activin A and regulates myofibroblast differentiation.

The Journal of Biological Chemistry
Sergio CarracedoDonald Gullberg

Abstract

Fibrotic tissue is characterized by an overabundance of myofibroblasts. Thus, understanding the factors that induce myofibroblast differentiation is paramount to preventing fibrotic healing. Previous studies have shown that mechanical stress derived from the integrin-mediated interaction between extracellular matrix and the cytoskeleton promotes myofibroblast differentiation. Integrin alpha11beta1 is a collagen receptor on fibroblasts. To determine whether alpha11beta1 can act as a mechanosensor to promote the myofibroblast phenotype, mouse embryonic fibroblasts and human corneal fibroblasts were utilized. We found that alpha11 mRNA and protein levels were up-regulated in mouse embryonic fibroblasts grown in attached three-dimensional collagen gels and conversely down-regulated in cells grown in floating gels. alpha11 up-regulation could be prevented by manually detaching the collagen gels or by cytochalasin D treatment. Furthermore, SB-431542, an inhibitor of signaling via ALK4, ALK5, and ALK7, prevented the up-regulation of alpha11 and the concomitant phosphorylation of Smad3 under attached conditions. In attached gels, TGF-beta1 was secreted in its inactive form but surprisingly not further activated, thus not influencing al...Continue Reading

References

Nov 1, 1991·The Journal of Investigative Dermatology·P DelvoyeC M Lapière
Feb 1, 1990·Experimental Cell Research·D GullbergK Rubin
Dec 1, 1994·The Journal of Cell Biology·R Chiquet-EhrismannM Chiquet
Jan 15, 1996·Molecular and Cellular Endocrinology·J P de WinterA J van den Eijnden-van Raaij
Nov 12, 1996·Biochemical and Biophysical Research Communications·E OhgaY Ouchi
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·R J Pelham, Y l Wang
May 30, 1998·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·A ShimizuK Miyazono
Jul 29, 1998·The Journal of Cell Biology·A PozziH A Gardner
Aug 30, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·B EckesT Krieg
Jul 27, 2001·The Journal of Biological Chemistry·D KesslerB Eckes
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 11, 2002·Nature Cell Biology·Martin A Schwartz, Mark H Ginsberg
May 4, 2002·Nature Reviews. Molecular Cell Biology·James J TomasekRobert A Brown
May 15, 2002·Immunology Letters·Aristidis MoustakasCarl Henrik Heldin
Feb 13, 2003·Biochimica Et Biophysica Acta·Martin FlückMatthias Chiquet
Apr 26, 2003·Matrix Biology : Journal of the International Society for Matrix Biology·Matthias ChiquetMartin Flück
Jun 17, 2003·Molecular Biology of the Cell·Boris HinzChristine Chaponnier
Feb 24, 2004·Molecular Pharmacology·Stacey DaCosta ByfieldAnita B Roberts
Jul 21, 2004·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Kate A LygoeMark P Lewis
Sep 7, 2004·The International Journal of Developmental Biology·Alexis DesmoulièreGiulio Gabbiani
Sep 20, 2005·Cancer Cell·Matthew J PaszekValerie M Weaver
Sep 29, 2005·Journal of Cell Science·Chee Ping NgMelody A Swartz
Nov 23, 2005·Matrix Biology : Journal of the International Society for Matrix Biology·Ning LuDonald Gullberg
Jan 27, 2006·The American Journal of Pathology·Yoshihide AsanoKunihiko Tamaki
Mar 31, 2006·Nature Reviews. Cancer·Raghu Kalluri, Michael Zeisberg
Jun 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Praveen R AranyAnita B Roberts
Aug 25, 2006·American Journal of Physiology. Cell Physiology·Joseph M MianoKeigi Fujiwara
Aug 25, 2006·Molecular Biology of the Cell·A Wayne OrrMartin A Schwartz
Sep 16, 2006·The Journal of Investigative Dermatology·Manon C ZweersBeate Eckes
Oct 31, 2006·The Journal of Investigative Dermatology. Symposium Proceedings·Beate EckesThomas Krieg
Feb 15, 2007·The Journal of Investigative Dermatology·Boris Hinz
Apr 11, 2007·Molecular and Cellular Biology·Svetlana N PopovaDonald Gullberg
May 26, 2007·The American Journal of Pathology·Boris HinzGiulio Gabbiani
Jun 20, 2007·Experimental Dermatology·Mara FumagalliCarlotta Castagnoli
Jun 22, 2007·Acta Physiologica·S N PopovaD Gullberg

❮ Previous
Next ❯

Citations

Aug 14, 2012·Archives of Dermatological Research·Chenyu HuangRei Ogawa
Feb 15, 2014·Advances in Wound Care·Susan W VolkArdeshir Bayat
Aug 8, 2012·Cardiovascular Research·Ilana Talior-VolodarskyChristopher A McCulloch
Mar 31, 2012·American Journal of Respiratory Cell and Molecular Biology·Xiangwei HuangYong Zhou
May 8, 2013·Cellular and Molecular Life Sciences : CMLS·Ping KongNikolaos G Frangogiannis
Mar 8, 2014·Journal of Cell Communication and Signaling·Sunil K Parapuram, William Hodge
Nov 28, 2013·Antioxidants & Redox Signaling·Alessandro Siani, Nicola Tirelli
Feb 22, 2011·Annual Review of Plant Biology·Vincent MirabetOlivier Hamant
Dec 11, 2014·Advances in Wound Care·Leeni KoivistoHannu Larjava
Feb 15, 2012·Connective Tissue Research·Chenyu Huang, Rei Ogawa
Feb 13, 2016·Frontiers in Physiology·Shuang LiangDavid A Brenner
Oct 6, 2012·Seminars in Cell & Developmental Biology·Victor W WongGeoffrey C Gurtner
Dec 19, 2015·Cellular and Molecular Life Sciences : CMLS·Ian A DarbyAlexis Desmoulière
Jan 31, 2015·The Journal of Investigative Dermatology·Jan-Niklas SchulzDonald Gullberg
Nov 30, 2012·The Journal of Investigative Dermatology·Shangxi Liu, Andrew Leask
Mar 4, 2015·Cell Adhesion & Migration·Jiří Kanta
Aug 8, 2015·Cardiovascular Research·He HuangCongxin Huang
Jun 25, 2015·Nature Reviews. Cancer·Holly E BarkerKevin J Harrington
Jul 15, 2016·BioMed Research International·Joanna RosińczukMirosław Sopel
Jun 29, 2016·Cell and Tissue Research·Nuno Miranda Coelho, Christopher A McCulloch
May 24, 2016·Current Opinion in Cell Biology·Jennifer Alexander, Edna Cukierman
Nov 18, 2017·Experimental & Molecular Medicine·Ruchi BansalJai Prakash
Feb 10, 2016·Journal of Cell Science·Cédric Zeltz, Donald Gullberg
Jul 26, 2012·Annals of Neurology·Michael Y LinRanjan Gupta
Jun 26, 2014·Journal of Cellular Physiology·Ilana Talior-VolodarskyChristopher A McCulloch
Sep 13, 2014·Journal of Cellular Physiology·Francisco J BlancoCarmelo Bernabeu
Sep 26, 2015·Journal of Cellular Biochemistry·Alexandra GrellaTanja Dominko
Jan 5, 2011·Cell Proliferation·C E SarrafM Eastwood
Sep 22, 2012·The Journal of Pathology·Franco KlingbergEric S White
May 21, 2016·American Journal of Physiology. Heart and Circulatory Physiology·Robert A CivitareseKim A Connelly
Jul 10, 2019·The Journal of Clinical Investigation·Irina PrimacAgnès Noel
Oct 13, 2019·The Journal of Pathology. Clinical Research·Hilde Ytre-Hauge SmelandLars A Akslen

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.