The filamentous fungus Aspergillus niger contains two "differentially regulated" trehalose-6-phosphate synthase-encoding genes, tpsA and tpsB.

The Journal of Biological Chemistry
M F Wolschek, C P Kubicek

Abstract

Two genes encoding trehalose-6-phosphate synthase were cloned from Aspergillus niger. tpsA was cloned using the Saccharomyces cerevisiae GGS1/TPS1 gene as a probe. It encodes a 517-amino acid polypeptide with 64-70% similarity to trehalose-6-phosphate synthase of S. cerevisiae, Kluyveromyces lactis, and Schizosaccharomyces pombe. Its transcription occurs constitutively and is enhanced on carbon-derepressing carbon sources, coinciding with the presence of a CreA-binding nucleotide motif in the 5'-noncoding region of tpsA. Disruption of tpsA only weakly reduces growth on glucose, and neither influences the glucose induction of a low affinity glucose permease nor interferes with the catabolite repression of a pectinase; it causes reduced the heat tolerance of conidia. tpsB was cloned by a polymerase chain reaction-based strategy. Its 480 amino acid sequence showed 76.5% identity to tpsA. Its transcription was hardly detectable at ambient temperatures but was enhanced strongly upon heat shock, which agrees with the presence of several copies of a C4T stress-responsive element in its 5'-upstream sequences. Hence the function of yeast GGS1/TPS1 has been split into two differentially regulated genes in A. niger, of which none appears ...Continue Reading

References

Nov 1, 1991·Molecular and Cellular Biology·C E Dowzer, J M Kelly
Jan 1, 1991·Proteins·G D SchulerD J Lipman
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Oct 1, 1990·Antonie van Leeuwenhoek·A Wiemken
Dec 1, 1987·Journal of Bacteriology·T HottigerA Wiemken
Jan 1, 1974·Advances in Carbohydrate Chemistry and Biochemistry·A D Elbein
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M M YeltonW E Timberlake
Mar 1, 1984·Microbiological Reviews·J M Thevelein
Jan 1, 1995·Trends in Biochemical Sciences·J M Thevelein, S Hohmann
Nov 27, 1995·FEBS Letters·J StraussC P Kubicek
Jan 1, 1953·Advances in Genetics·G PONTECORVOA W J BUFTON

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Citations

Nov 7, 2006·Applied Microbiology and Biotechnology·L K LiangZ M Chi
Aug 13, 2003·Eukaryotic Cell·George J G RuijterJaap Visser
May 5, 2010·Infection and Immunity·Nadia Al-BaderDonald C Sheppard
Apr 17, 2003·Applied Microbiology and Biotechnology·Levente Karaffa, Christian P Kubicek
Jul 1, 2004·Critical Reviews in Biotechnology·Zhonghu BaiBrian McNeil
May 2, 2008·Fungal Genetics and Biology : FG & B·Kazutoshi SakamotoOsamu Akita
May 17, 2007·The New Phytologist·Aurora OcónNatalia Requena
Mar 2, 2013·Studies in Mycology·P KrijgsheldH A B Wösten
Jan 22, 2004·FEMS Yeast Research·Carlos Gancedo, Carmen-Lisset Flores
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