The final step in the phage infection cycle: the Rz and Rz1 lysis proteins link the inner and outer membranes.

Molecular Microbiology
Joel D BerryRyland Young

Abstract

Bacteriophage lambda has four adjacent genes -S, R, Rz and Rz1- dedicated to host cell lysis. While S, encoding the holin and antiholin, and R, encoding the endolysin, have been intensively studied, the products of Rz and Rz1 have not been characterized at either the structural or functional levels. Rz1 is an outer membrane lipoprotein and our results indicate that Rz is a type II signal anchor protein. Here we present evidence that an Rz-Rz1 complex that spans the periplasm carries out the final step in the process of host lysis. These results are discussed in terms of a model where endolysin-mediated degradation of the cell wall is a prerequisite for conformational changes in the Rz-Rz1 complex leading to the juxtaposition and fusion of the IM and OM. Fusion of the two membranes removes the last physical barrier to efficient release of progeny virions.

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Citations

Jul 23, 2013·Applied Microbiology and Biotechnology·Yongqiang GaoGuang Zhao
Oct 29, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ting PangRy Young
Dec 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·Xinyao Liu, Roy Curtiss
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jill S DeweyRy Young
Oct 27, 2009·Applied and Environmental Microbiology·Chia-Ni LeeYi-Hsiung Tseng
May 17, 2011·Journal of Bacteriology·Lauranell H BurchJohn W Drake
Nov 10, 2009·Journal of Bacteriology·Elizabeth J SummerCarlos F Gonzalez
Aug 21, 2012·Journal of Bacteriology·Joel BerryRy Young
Oct 27, 2010·BMC Genomics·Karlene H LynchJonathan J Dennis
Jul 18, 2012·BMC Genomics·Darren L SmithHeather E Allison
Oct 12, 2012·BMC Genomics·Jason J GillRy Young
Aug 4, 2011·BMC Microbiology·John J Dennehy, Ing-Nang Wang
Mar 4, 2014·The Journal of Microbiology·Ryland Young
May 5, 2016·Bacteriophage·Gail E Christie, Richard Calendar
Oct 12, 2013·Current Opinion in Microbiology·Ry Young
Apr 15, 2015·Proceedings of the National Academy of Sciences of the United States of America·Manoj RajaureRy Young
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Feb 27, 2018·Virus Genes·K S Sritha, Sarita G Bhat
May 5, 2017·Journal of Virology·Jesse CahillRy Young
Nov 15, 2018·Viruses·Alexander B CarstensLars H Hansen
Jan 29, 2014·Virology Journal·Johannes WittmannChristine Rohde
Jan 4, 2017·G3 : Genes - Genomes - Genetics·Jesse CahillRy Young
Aug 17, 2018·Viruses·Maria João Catalão, Madalena Pimentel
Aug 25, 2010·Protein Science : a Publication of the Protein Society·Joel BerryRy Young
Sep 17, 2018·BMC Bioinformatics·Rohit KongariRy Young
Aug 24, 2018·Journal of Virology·Rohit KongariRy Young

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