The fine art of integral membrane protein crystallisation

Methods : a Companion to Methods in Enzymology
James BirchIsabel Moraes

Abstract

Integral membrane proteins are among the most fascinating and important biomolecules as they play a vital role in many biological functions. Knowledge of their atomic structures is fundamental to the understanding of their biochemical function and key in many drug discovery programs. However, over the years, structure determination of integral membrane proteins has proven to be far from trivial, hence they are underrepresented in the protein data bank. Low expression levels, insolubility and instability are just a few of the many hurdles one faces when studying these proteins. X-ray crystallography has been the most used method to determine atomic structures of membrane proteins. However, the production of high quality membrane protein crystals is always very challenging, often seen more as art than a rational experiment. Here we review valuable approaches, methods and techniques to successful membrane protein crystallisation.

Citations

Jun 2, 2018·PloS One·Octavio Martínez, M Humberto Reyes-Valdés
Nov 23, 2018·Biochemical Society Transactions·Kailene S SimonSarah C Lee
Mar 5, 2019·F1000Research·James P Allen
May 30, 2019·International Journal of Molecular Sciences·Tristan O C KwanIsabel Moraes
Feb 26, 2019·Future Medicinal Chemistry·María Julia AmundarainAlejandro Giorgetti
Nov 11, 2020·Biochemical Society Transactions·Tristan O C KwanIsabel Moraes
Dec 20, 2020·The FEBS Journal·Ali A Kermani
Jan 13, 2021·The Journal of Membrane Biology·Zenia MotiwalaKiran Kulkarni
Nov 5, 2019·Protein Expression and Purification·Daniel N WisemanAlan D Goddard
Nov 20, 2020·Biology·James BirchAndrew Quigley
Dec 31, 2020·International Journal of Molecular Sciences·Johannes Thoma, Björn M Burmann
Dec 6, 2020·Nature Communications·Jorge Roel-TourisAlexandre M J J Bonvin
May 18, 2020·Biochimica Et Biophysica Acta. Biomembranes·Gaddafi I Danmaliki, Peter M Hwang

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