PMID: 6162359Sep 1, 1980Paper

The fine structure of "dividers" and "non-dividers" in phase II human glial cell cultures

Acta Pathologica Et Microbiologica Scandinavica. Section A, Pathology
E BlomquistB A Fredriksson

Abstract

A method is described which allows a comparison in the transmission electron microscope (TEM) of cells with different remaining proliferative capacity from one and the same culture. The method takes advantage of a mini-cloning technique employing hapatotactic palladium islands in combination with micro-dissection and preparation for TEM of islands carrying various numbers of cells after 10 days in culture, when all miniclones have become density dependent growth inhibited. By means of this technique non-dividers were compared with miniclones of dividers composed of five to eight cells originating from single cells. Moreover, large, immotile cells without peripheral ruffling activity, known to be non-dividers, were compared with small, ruffling cells, known to be dividers, in the reflection-interference mode in sparse cultures of living cells, and in the TEM mode as whole cell preparations after critical point drying of cells cultured on formvar-coated, gold EM-grids. Non-dividers proved to contain a moderate number of residual bodies, well developed Golgi areas, and often branched or circular mitochondria; they were thinly spread over the substratum with many focal points of contact, and large areas of close apposition between ...Continue Reading

References

Feb 1, 1978·Experimental Cell Research·B Westermark
Jul 1, 1979·The Journal of Cell Biology·J J Wolosewick, K R Porter
Jan 1, 1979·Gerontology·K Kontermann, K Bayreuther
Aug 1, 1978·Mechanisms of Ageing and Development·V P Collins, U T Brunk
Nov 1, 1976·The American Journal of Anatomy·J J Wolosewick, K R Porter
Jul 1, 1974·The Journal of Cell Biology·J R Smith, L Hayflick
Mar 15, 1973·Experimental Cell Research·A Harris
Dec 1, 1969·Journal of Cellular Physiology·G S Merz, J D Ross
Dec 1, 1969·Experimental Cell Research·J PonténR Hugosson
Dec 1, 1961·Experimental Cell Research·L HAYFLICK, P S MOORHEAD

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Acta Pathologica Et Microbiologica Scandinavica. Section A, Pathology
E BlomquistB Westermark
Acta Pathologica Et Microbiologica Scandinavica. Section A, Pathology
E BlomquistB Westermark
Acta Pathologica Et Microbiologica Scandinavica. Section A, Pathology
V P CollinsB Westermark
© 2021 Meta ULC. All rights reserved