The first anionic defensin from amphibians

Amino Acids
Lin WeiHailong Yang

Abstract

A variety of antimicrobial peptides against infection have been identified from the skin of amphibians. However, knowledge on amphibian defensins is very limited. A novel anionic defensin designated PopuDef was purified from the skin of tree frog Polypedates puerensis, and the cDNA encoding PopuDef precursor was cloned from the skin cDNA library. The amino acid sequence of PopuDef (net charge: -2, pI: 4.75) shared the highest identity of 57 % (25/44) with the salamander defensin CFBD-1 (net charge: 0, pI: 6.14) from urodela amphibians. PopuDef showed moderate antimicrobial activities against P. aeruginosa and S. aureus (MICs are 19.41 and 17.25 μM, respectively), and relatively weak activities against E. coli and B. subtilis (MICs are 38.82 and 43.14 μM, respectively). Tissue distribution analysis indicated that relatively high expression level of PopuDef mRNA was observed in immune-related tissues including skin, gut, lung and spleen. Furthermore, the expression level of PopuDef was significantly upregulated in these tissues after tree frogs were infected with different bacteria strains mentioned above. Interestingly, the induction of PopuDef challenged with E. coli or B. subtilis, which was less sensitive to PopuDef, was much...Continue Reading

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Citations

Jul 24, 2015·Biomolecular Concepts·Małgorzata Cytryńska, Agnieszka Zdybicka-Barabas
Jul 27, 2018·The Biochemical Journal·Jing WuLixian Mu
Mar 15, 2020·International Journal of Molecular Sciences·Aneta Sowa-JasiłekMałgorzata Cytryńska
Mar 11, 2021·Biochemical and Biophysical Research Communications·Caifen GuoBaiyu Zhang
Dec 10, 2019·New Biotechnology·Meron GhideyChristopher Michel Kearney

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