The first application of terephthalate fluorescence for highly selective detection of hydroxyl radicals in thylakoid membranes

Functional Plant Biology : FPB
Iva Šnyrychová, Éva Hideg

Abstract

Possibilities and limitations of the detection of hydroxyl radicals via the conversion of terephthalate (TPA) into the strongly fluorescent hydroxyterephthalate were investigated in order to adapt this method for chlorophyll-containing samples. Using model chemical sources of various reactive oxygen species, we confirmed that TPA detects hydroxyl radicals very sensitively, but is not reactive to either hydrogen peroxide or superoxide radicals. As a new result, we showed that the conversion of TPA to hydroxyterephthalate cannot be induced by singlet oxygen, which may be produced in photosynthetic systems under stress. Until now, the TPA method has not been used in photosynthesis research, so necessary adaptations to minimise the effects of chlorophyll and buffering sugars on hydroxyl radical detection were also explored and optimal conditions for using the method in thylakoid preparations are suggested. Anticipating further plant physiology applications, usefulness of the TPA method was tested in a wider range of pH than reported earlier. To demonstrate that this simple and highly specific method can be used as an alternative approach for the detection of hydroxyl radicals in plant samples, we measured these radicals in isolated...Continue Reading

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Citations

Feb 6, 2019·Scientific Reports·Gyula CzégényÉva Hideg
Feb 1, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jiaqi NiYi Liu
Jan 1, 2008·Functional Plant Biology : FPB·Iva ŠnyrychováÉva Hideg
Dec 12, 2018·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Anikó MátaiÉva Hideg
Nov 4, 2010·International Journal of Food Sciences and Nutrition·Silviya StoyanovaWim Van Den Ende
Jan 22, 2020·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Susanne NeugartÅke Strid

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