The first caldesmon-like protein in higher plants

Biochemical and Biophysical Research Communications
K KrauzeR Dabrowska

Abstract

Using anti-caldesmon polyclonal and monoclonal (raised against the N-terminal fragment of chicken gizzard caldesmon) antibodies, a plant caldesmon-like protein, 107 kDa as determined by SDS-gel electrophoresis, has been identified based on Western blotting of total extracts of Ornithogalum virens pollen tubes. Biochemical investigations showed common properties of this protein with animal caldesmon--it binds to actin and, in a Ca(2+)-dependent manner, to calmodulin. In contrast to animal caldesmon, this plant cell counterpart is relatively resistant to proteolysis by endogenous proteases and sensitive to heat treatment. Our results show the presence of a caldesmon-like protein in higher plants for the first time.

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Citations

Nov 1, 2001·Annual Review of Cell and Developmental Biology·P K HeplerA Y Cheung
Dec 6, 2001·Protoplasma·L Vidali, P K Hepler
Dec 23, 1999·Current Opinion in Plant Biology·V E Franklin-Tong
Mar 31, 2009·European Journal of Cell Biology·Christine R FaulknerRobyn L Overall

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