The first cytokine sequence within cartilaginous fish: IL-1 beta in the small spotted catshark (Scyliorhinus canicula)

The Journal of Immunology : Official Journal of the American Association of Immunologists
Steve BirdChris J Secombes

Abstract

Cartilaginous fish are considered the most primitive living jawed vertebrates with a complex immune system typical of all jawed vertebrates. Cytokine homologs are found within jawless and bony fish, although no cytokine or cytokine receptor genes have been sequenced in cartilaginous fish. In this study the complete coding sequence of the small spotted catshark (Scyliorhinus canicula) IL-1beta gene is presented that contains a short 5' untranslated region (54 bp), a 903-bp open reading frame, a 379-bp 3' untranslated region, a polyadenylation signal, and eight mRNA instability motifs. The predicted translation (301 amino acids) has highest identity to trout IL-1beta (31.7%), with greatest homology within the putative 12 beta-sheets. The IL-1 family signature is also present, but there is no apparent signal peptide. As with other nonmammalian IL-1beta sequences, the IL-1-converting enzyme cut site is absent. Expression of the IL-1beta transcript is detectable by RT-PCR in the spleen and testes, induced in vivo with LPS. Furthermore, a 7-fold increase of transcript levels in splenocytes incubated for 5 h with LPS was seen. The genomic organization comprises six exons and five introns with highest homology seen in exons encoding th...Continue Reading

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Citations

Apr 17, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Shuangshuang LuChun Xia
Aug 2, 2005·Marine Biotechnology·Francesco BuonocoreGiuseppe Scapigliati
Jun 26, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tiehui WangChristopher J Secombes
Nov 7, 2019·Nature Reviews. Immunology·Petr BrozFeng Shao
Dec 12, 2012·PloS One·Marta I R ReisNuno M S Dos Santos
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May 28, 2016·Biology·Jun Zou, Christopher J Secombes
Aug 2, 2003·Fish & Shellfish Immunology·Yuuki InoueTeruyuki Nakanishi
Mar 9, 2005·Developmental and Comparative Immunology·Meagan E PressleyCarol H Kim
Feb 3, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Dae-Sim LeeHyun Do Jeong
Nov 18, 2008·Developmental and Comparative Immunology·Anders E ØstergaardChristopher J Secombes
Jan 1, 2008·CSH Protocols·Marion CoolenSylvie Mazan

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