Abstract
A key factor in the characterization of peptide transmitters used in neuronal signaling is the correct elucidation of post-translational modifications, especially as they are often required to confer biological activity. A rare carboxylation modification is described on the D-peptide from the insulin prohormone in the sea slug, Aplysia californica. Using liquid chromatography purification coupled with electrospray ionization and nanoelectrospray ionization-ion trap-mass spectrometry (ESI- and nanoESI-MS), the presence of this D-peptide within Aplysia insulin (AI)-producing neurons is confirmed. Further detailed mass spectrometric analyses demonstrate that the Aplysia insulin D-peptide is carboxylated on the single glutamate residue within the sequence. This gamma-carboxy D-peptide, along with other identified AI-related peptides, is secreted from the central nervous system in response to ionophore stimulation, thus suggesting a signaling role within the nervous system. Although carboxylated peptides have been described previously, the Aplysia gamma-carboxy D-peptide appears to be the first reported carboxylated neuropeptide.
References
Aug 1, 1992·The Journal of Clinical Endocrinology and Metabolism·W H Daughaday, B Trivedi
Jan 23, 1990·Biochimica Et Biophysica Acta·V W Hylka, D S Straus
May 1, 1987·Endocrinology·V W HylkaD S Straus
Jan 1, 1984·Methods in Enzymology·G L Nelsestuen
Oct 1, 1980·Analytical Biochemistry·P V HauschkaP M Gallop
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·W H DaughadayR C Baxter
May 1, 1993·The Journal of Clinical Endocrinology and Metabolism·F LiuR L Hintz
Jul 1, 1996·Endocrinology·C Q YangJ F Perdue
Mar 1, 1996·Protein Science : a Publication of the Protein Society·T NakamuraA L Burlingame
Apr 21, 1997·FEBS Letters·T B StanleyP K Bandyopadhyay
Feb 14, 1998·Progress in Neurobiology·A B SmitW P Geraerts
Jul 11, 1998·The Journal of Biological Chemistry·S J DuguayD F Steiner
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·A C RigbyJ Stenflo
Jul 29, 1999·Analytical Chemistry·K R ClauserA L Burlingame
Sep 22, 1999·European Journal of Biochemistry·A G CraigB M Olivera
Oct 12, 1999·Analytical Chemistry·N L KelleherC T Walsh
Feb 19, 2000·Journal of Mass Spectrometry : JMS·D E KalumeP Roepstorff
Dec 9, 2000·The Journal of Biological Chemistry·C S WalkerP K Bandyopadhyay
May 8, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·M StarkJ Johansson
Jan 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Pradip K BandyopadhyayBaldomero M Olivera
Nov 13, 2004·Analytical Chemistry·Jennifer A Jakubowski, Jonathan V Sweedler
Mar 15, 2005·Analytical Chemistry·Nathan G HatcherJonathan V Sweedler
Jun 22, 2005·Biochemistry·Mark A BrownBruce Furie
Citations
Aug 23, 2008·Proceedings of the National Academy of Sciences of the United States of America·Nathan G HatcherJonathan V Sweedler
Dec 24, 2011·PloS One·Ping YinJonathan V Sweedler
Aug 25, 2015·General and Comparative Endocrinology·Samuel D RobinsonHelena Safavi-Hemami
Jul 19, 2008·Annual Review of Analytical Chemistry·Lingjun Li, Jonathan V Sweedler
Jun 20, 2014·Advanced Materials·Wei ZengXiao-Ming Tao
Oct 26, 2007·Lab on a Chip·Kyubong JoJonathan V Sweedler
Aug 28, 2009·Radiation Research·Juliann G KiangNeil G Agravante
Nov 16, 2007·Journal of Neurophysiology·Nathan G Hatcher, Jonathan V Sweedler
Mar 3, 2007·The Biochemical Journal·Carolina Möller, Frank Marí
Feb 5, 2021·ACS Chemical Biology·Md Shadman Ridwan AbidJames W Checco
Aug 19, 2008·Analytical Chemistry·Stanislav S Rubakhin, Jonathan V Sweedler
Mar 18, 2008·Analytical Chemistry·Michael A EwingJonathan V Sweedler