The first human epitope map of the alphaviral E1 and E2 proteins reveals a new E2 epitope with significant virus neutralizing activity.

PLoS Neglected Tropical Diseases
Ann R HuntCarol D Blair

Abstract

Venezuelan equine encephalitis virus (VEEV) is responsible for VEE epidemics that occur in South and Central America and the U.S. The VEEV envelope contains two glycoproteins E1 (mediates cell membrane fusion) and E2 (binds receptor and elicits virus neutralizing antibodies). Previously we constructed E1 and E2 epitope maps using murine monoclonal antibodies (mMAbs). Six E2 epitopes (E2(c,d,e,f,g,h)) bound VEEV-neutralizing antibody and mapped to amino acids (aa) 182-207. Nothing is known about the human antibody repertoire to VEEV or epitopes that engage human virus-neutralizing antibodies. There is no specific treatment for VEE; however virus-neutralizing mMAbs are potent protective and therapeutic agents for mice challenged with VEEV by either peripheral or aerosol routes. Therefore, fully human MAbs (hMAbs) with virus-neutralizing activity should be useful for prevention or clinical treatment of human VEE. We used phage-display to isolate VEEV-specific hFabs from human bone marrow donors. These hFabs were characterized by sequencing, specificity testing, VEEV subtype cross-reactivity using indirect ELISA, and in vitro virus neutralization capacity. One E2-specific neutralizing hFAb, F5n, was converted into IgG, and its bind...Continue Reading

References

Jul 1, 1975·Journal of Virology·C H von Bonsdorff, S C Harrison
Nov 1, 1978·Journal of Virology·C H von Bonsdorff, S C Harrison
Sep 1, 1976·Journal of Virology·J F ObijeskiE L Palmer
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D R BurtonR A Lerner
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A S KangR A Lerner
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y T ChenH P Moore
Jan 1, 1994·Clinical Microbiology Reviews·C H Calisher
Apr 1, 1997·The Journal of Infectious Diseases·F RivasT Tsai
Jun 1, 1997·The American Journal of Tropical Medicine and Hygiene·D M WattsC G Hayes
Feb 6, 1998·The American Journal of Tropical Medicine and Hygiene·D M WattsC G Hayes
Feb 6, 1998·The American Journal of Tropical Medicine and Hygiene·M S ObersteJ F Smith
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·C RaderC F Barbas
Sep 7, 2001·Annual Review of Microbiology·R J Hawley, E M Eitzen
Dec 12, 2001·Journal of Virology·Cristina de Carvalho NicacioEwa Björling
Jun 21, 2002·The American Journal of the Medical Sciences·Michael S BronzeRonald A Greenfield
Oct 22, 2002·Journal of Virology·Wei ZhangMichael G Rossmann
Apr 23, 2003·Virology·Joachim KochEkkehard K F Bautz

❮ Previous
Next ❯

Citations

Nov 8, 2011·Biosecurity and Bioterrorism : Biodefense Strategy, Practice, and Science·Aruna SampathJoseph C Larsen
Mar 21, 2013·Applied Microbiology and Biotechnology·Sun EnChengWu DongLai
Apr 30, 2013·Current Opinion in Virology·Marie-Christine VaneyFélix A Rey
Jul 25, 2012·Veterinary Immunology and Immunopathology·J ZhaoD L Wu
Aug 4, 2011·Journal of Invertebrate Pathology·Stefan W Metz, Gorben P Pijlman
Feb 13, 2014·MAbs·Birgit HülsewehMichael Hust
Jun 9, 2016·Protein Engineering, Design & Selection : PEDS·Andrew C R Martin, Anthony R Rees
Jun 13, 2014·Journal of Virology·Jason PortaMichael G Rossmann
Nov 30, 2011·MAbs·Jeffrey W FroudePhilippe Thullier
May 21, 2016·Proteomics. Clinical Applications·Philipp KuhnMichael Hust
Sep 24, 2019·Antibodies·Jinny L LiuGeorge P Anderson
May 1, 2021·Microorganisms·Anthony Torres-RuestaLisa F P Ng
Jul 27, 2021·Frontiers in Cellular and Infection Microbiology·Kristian Daniel Ralph RothMichael Hust
Aug 27, 2021·The Journal of General Virology·Israel Guerrero-ArgueroBrett E Pickett

❮ Previous
Next ❯

Datasets Mentioned

BETA
HM047070

Methods Mentioned

BETA
phage-display
phage display
ELISA
PCR
transfection
ELISAs
glycosylation
reverse transcription PCR

Software Mentioned

DNAstar

Related Concepts

Related Feeds

Antibody Repertoire Diversity

Antibody repertoire diversity and its role during natural infection is a prerequisite for molecular and structural elucidation of functionally protective immunity. Discover the latest insights into antibody diversity here.

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.