The first in vivo multiparametric comparison of different radiation exposure biomarkers in human blood

PloS One
A TichyC Badie

Abstract

The increasing risk of acute large-scale radiological/nuclear exposures of population underlines the necessity of developing new, rapid and high throughput biodosimetric tools for estimation of received dose and initial triage. We aimed to compare the induction and persistence of different radiation exposure biomarkers in human peripheral blood in vivo. Blood samples of patients with indicated radiotherapy (RT) undergoing partial body irradiation (PBI) were obtained soon before the first treatment and then after 24 h, 48 h, and 5 weeks; i.e. after 1, 2, and 25 fractionated RT procedures. We collected circulating peripheral blood from ten patients with tumor of endometrium (1.8 Gy per fraction) and eight patients with tumor of head and neck (2.0-2.121 Gy per fraction). Incidence of dicentrics and micronuclei was monitored as well as determination of apoptosis and the transcription level of selected radiation-responsive genes. Since mitochondrial DNA (mtDNA) has been reported to be a potential indicator of radiation damage in vitro, we also assessed mtDNA content and deletions by novel multiplex quantitative PCR. Cytogenetic data confirmed linear dose-dependent increase in dicentrics (p < 0.01) and micronuclei (p < 0.001) in peri...Continue Reading

References

Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·F M Yakes, B Van Houten
May 11, 2001·Nature·R S ScottG K Matsushima
Apr 30, 2002·Strahlentherapie und Onkologie : Organ der Deutschen Röntgengesellschaft ... [et al]·Eduard GershkevitshKlaus-Rüdiger Trott
Jul 29, 2004·FEBS Letters·Sheela PrithivirajsinghWilliam A Brock
Feb 14, 2007·International Journal of Hygiene and Environmental Health·Milena CernáRůzena Kubínová
May 6, 2008·Journal of Biomedical Science·M Ahmad Chaudhry
Jun 24, 2008·International Journal of Radiation Oncology, Biology, Physics·Sunirmal Paul, Sally A Amundson
Nov 12, 2010·International Journal of Radiation Biology·Sylwia KabacikChristophe Badie
Feb 1, 2013·International Journal of Radiation Biology·Grainne ManningChristophe Badie
Jul 19, 2013·Radiation Research·K RothkammM Abend
Jul 28, 2013·Radiation Research·C BadieM Abend
Jan 23, 2016·Radiation and Environmental Biophysics·Antonella BertucciDavid J Brenner
Apr 2, 2016·Scientific Reports·Daniel R MartinStanley M Stevens
Jul 13, 2016·Clinical Oncology : a Journal of the Royal College of Radiologists·C BadieU Kulka
Mar 3, 2017·Genome Integrity·Dimphy ZeegersM Prakash Hande
Jan 14, 2018·Scientific Reports·Gráinne O'BrienChristophe Badie

❮ Previous
Next ❯

Citations

Jun 23, 2018·International Journal of Radiation Biology·Melis KarabulutogluChristophe Badie
Dec 13, 2019·International Journal of Radiation Biology·Gabriela KultovaAlena Myslivcova-Fucikova
May 29, 2019·Radiation Protection Dosimetry·Stanislav PolozovChristophe Badie
Sep 10, 2020·Journal of Personalized Medicine·Daijiro KobayashiTatsuya Ohno
Nov 5, 2019·Frontiers in Immunology·Valéria Bumiller-BiniAngelica Beate Winter Boldt
Oct 6, 2020·Journal of Applied Toxicology : JAT·Andrea MalkovaZdenek Fiala
Sep 16, 2020·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Vanesa BiolattiIrene L Ibañez
Oct 30, 2020·International Journal of Molecular Sciences·Lourdes Cruz-GarciaChristophe Badie
Apr 8, 2021·International Journal of Radiation Biology·Farah NasserChristophe Badie
Oct 27, 2021·International Journal of Radiation Biology·Eliseos J MucakiPeter K Rogan

❮ Previous
Next ❯

Methods Mentioned

BETA
flow-cytometry
chromosomal aberrations
PCR
flow cytometry
chromosomal aberration assay

Software Mentioned

Rotor
Eclipse
SPSS Statistics
Gene Q Series
Summit

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.