The first invertebrate NFIL3 transcription factor with role in immune defense identified from the Hong Kong oyster, Crassostrea hongkongensis

Developmental and Comparative Immunology
Jun LiZiniu Yu

Abstract

NFIL3 (nuclear factor interleukin 3-regulated) is a basic leucine zipper type transcription factor that mediates a variety of immune responses in vertebrates. However, the sequence information and function of NFIL3 homologs in invertebrates, especially mollusks, remains unknown. In the present study, the first NFIL3 homolog was identified in a marine mollusk, Crassostrea hongkongensis (designated as ChNFIL3), followed by its functional characterization. The full-length cDNA of ChNFIL3 is 2221 bp and consists of an open reading frame (ORF) of 1536 bp that encodes a polypeptide of 551 amino acids. Simple Modular Architecture Research Tool (SMART) analysis indicated that ChNFIL3 has two basic leucin zipper domains, similar to the other known NFIL3 family proteins. Tissue distribution analysis of NFIL3 in this mollusk revealed high expression in digestive glands and hemocytes. A significant induction in the mRNA level of ChNFIL3 was observed following bacterial stimulation. ChNFIL3 was found to be localized in the nucleus and over expression of ChNIFL3 led to upregulation of transcriptional activity of an NF-κB reporter gene in HEK 293T cells, indicating its role in innate immunity. Furthermore, addition of exogenous recombinant Ch...Continue Reading

References

Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·S IkushimaA T Look
Mar 21, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·F B Gao
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 17, 2005·Environmental Microbiology·Carla PruzzoLaura Canesi
Sep 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Euan LockhartJoAnne L Flynn
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Oct 3, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Franco SilvestrisFranco Dammacco
May 9, 2009·Developmental and Comparative Immunology·Ingrid SkjaevelandJorunn B Jørgensen
Dec 10, 2009·The Journal of Experimental Medicine·Shintaro KamizonoTak W Mak
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Masaki KashiwadaPaul B Rothman
May 12, 2010·Journal of Invertebrate Pathology·Rhiannon P KuchelNicole Vella
Nov 30, 2010·Molecular and Cellular Neurosciences·Harold D MacGillavryRonald E van Kesteren
Mar 9, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Taku KobayashiScott E Plevy
Apr 19, 2011·The EMBO Journal·Masaki KashiwadaPaul B Rothman
May 9, 2012·Developmental and Comparative Immunology·Fränze ProgatzkyJonathan R Lamb
Aug 13, 2014·The Journal of Experimental Medicine·Theresa L GeigerJoseph C Sun
Dec 24, 2014·Journal of Cellular Biochemistry·Kuan-Ho LinChih-Yang Huang

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