The first specific detection of a highly pathogenic avian influenza virus (H5N1) in Ivory Coast

Zoonoses and Public Health
E Couacy-HymannG Cattoli

Abstract

The Virology Laboratory of the Central Laboratory of Animal Diseases in Ivory Coast at Bingerville received samples of wild and domestic avian species between February and December 2006. An RT-PCR technique was used to test for avian influenza (AI) and highly pathogenic AI subtype viruses. Among 2125 samples, 16 were type A positive; of which, 12 were later confirmed to be H5N1. Fifteen of these 16 type A positive samples were inoculated into the chorioallantoic cavity of 11-day-old embryonated hens' eggs for virus isolation. Eight produced virus with hemagglutination titres from 1/64 to 1/512. The 4/16 M-RT-PCR positive samples, which were H5N1 negative, were shown to be H7 subtype negative. The diagnostic efficiency of the laboratory for the surveillance of H5N1 in Ivory Coast was demonstrated. The positive cases of H5N1 were from a sparrowhawk (Accipter nisus); live market poultry and in free-range poultry, where the mortality rate was approximately 20% (2/10) and 96.7% (29/30) respectively. Currently, investigations into intensive poultry farms have proved negative for H5N1. No human cases have been reported this time.

References

Jul 29, 1995·BMJ : British Medical Journal·D M FlemingP Litton
Jun 22, 2000·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·E StarickO Werner
Aug 3, 2001·Journal of Virological Methods·M S LeeH K Shieh
Apr 9, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Paul K S Chan
Jan 25, 2005·Emerging Infectious Diseases·Juthatip KeawcharoenYong Poovorawan
May 14, 2005·Emerging Infectious Diseases·Roongroje ThanawongnuwechYong Poovorawan
Sep 27, 2005·Journal of Virological Methods·Sunchai PayungpornYong Poovorawan
Jul 11, 2006·Nature·M F DucatezC P Muller
Jul 28, 2006·Nature·Erika Check
Jun 8, 2007·Emerging Infectious Diseases·Steven L SalzbergIlaria Capua
Jun 8, 2007·Emerging Infectious Diseases·Mariette F DucatezClaude P Muller
Jun 20, 2007·American Journal of Infection Control·Ji-Ming ChenYing-Xue Sun

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Citations

Aug 31, 2012·Emerging Infectious Diseases·Emmanuel Couacy-HymannMariette F Ducatez
Mar 15, 2016·The Journal of Veterinary Medical Science·Sachiko MoriguchiKoichi Goka
Jan 8, 2013·The Journal of Veterinary Medical Science·Kosuke SodaToshihiro Ito
Oct 30, 2020·Viruses·Susan A Shriner, J Jeffrey Root

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