The first structure of a glycoside hydrolase family 61 member, Cel61B from Hypocrea jecorina, at 1.6 A resolution.

Journal of Molecular Biology
Saeid KarkehabadiMats Sandgren

Abstract

The glycoside hydrolase (GH) family 61 is a long-recognized, but still recondite, class of proteins, with little known about the activity, mechanism or function of its more than 70 members. The best-studied GH family 61 member, Cel61A of the filamentous fungus Hypocrea jecorina, is known to be an endoglucanase, but it is not clear if this represents the main activity or function of this family in vivo. We present here the first structure for this family, that of Cel61B from H. jecorina. The best-quality crystals were formed in the presence of nickel, and the crystal structure was solved to 1.6 A resolution using a single-wavelength anomalous dispersion method with nickel as the source of anomalous scatter. Cel61B lacks a carbohydrate-binding module and is a single-domain protein that folds into a twisted beta-sandwich. A structure-aided sequence alignment of all GH family 61 proteins identified a highly conserved group of residues on the surface of Cel61B. Within this patch of mostly polar amino acids was a site occupied by the intramolecular nickel hexacoordinately bound in the solved structure. In the Cel61B structure, there is no easily identifiable carbohydrate-binding cleft or pocket or catalytic center of the types normal...Continue Reading

References

May 25, 1977·Journal of Molecular Biology·F C BernsteinM Tasumi
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Sep 15, 1995·Structure·G Davies, B Henrissat
Aug 2, 1996·Science·L Holm, C Sander
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·K KuhlsC P Kubicek
Dec 15, 1996·Structure·G J Kleywegt, T A Jones
Aug 15, 1997·Structure·C A OrengoJ M Thornton
Jan 20, 1998·Journal of Immunological Methods·D Siev
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Dec 12, 2001·European Journal of Biochemistry·J KarlssonF Tjerneld
Sep 28, 2002·Acta Crystallographica. Section D, Biological Crystallography·Thomas R Schneider, George M Sheldrick
Mar 4, 2003·Bioinformatics·Rodrigo LopezPetteri Jokinen
Jun 5, 2003·Chemical Communications : Chem Comm·R J P Williams
Jun 6, 2003·The Journal of Biological Chemistry·Pamela K ForemanMichael Ward
Jun 26, 2003·Nucleic Acids Research·Rodrigo LopezWarren Gish
Jun 28, 2003·FEMS Microbiology Reviews·Scott B Mulrooney, Robert P Hausinger
Oct 2, 2003·Bioscience, Biotechnology, and Biochemistry·Yukari HaraKiyoshi Ito
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Jun 24, 2004·Biochemistry·David P BarondeauElizabeth D Getzoff
Jul 1, 1996·Acta Crystallographica. Section D, Biological Crystallography·G J Kleywegt, T A Jones
Jul 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·A PerrakisV S Lamzin
May 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Gerard J KleywegtT Alwyn Jones
Dec 8, 2004·Current Opinion in Structural Biology·David P Barondeau, Elizabeth D Getzoff
Dec 14, 2004·The Journal of Biological Chemistry·Gustav Vaaje-KolstadDaan M F van Aalten
Dec 21, 2004·Nucleic Acids Research·Amos BairochLai-Su L Yeh
May 25, 2005·Progress in Biophysics and Molecular Biology·Elspeth F Garman, Geoffrey W Grime
Jun 3, 2005·The Journal of Biological Chemistry·Gustav Vaaje-KolstadVincent G H Eijsink
Oct 6, 2005·Acta Crystallographica. Section D, Biological Crystallography·Janet NewmanAnastassis Perrakis

❮ Previous
Next ❯

Citations

Feb 1, 2012·Applied Microbiology and Biotechnology·Anamika RayAndrew J Mort
Sep 3, 2013·Applied Microbiology and Biotechnology·Maria DimarogonaPaul Christakopoulos
Nov 6, 2010·Biomolecular NMR Assignments·Finn L AachmannGustav Vaaje-Kolstad
Aug 31, 2011·Proceedings of the National Academy of Sciences of the United States of America·R Jason QuinlanPaul H Walton
Nov 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Finn L AachmannGustav Vaaje-Kolstad
Oct 12, 2010·Science·Gustav Vaaje-KolstadVincent G H Eijsink
Apr 20, 2010·Applied and Environmental Microbiology·Amber Vanden WymelenbergDan Cullen
Apr 21, 2009·Applied and Environmental Microbiology·Amber Vanden WymelenbergDan Cullen
Mar 26, 2013·Applied and Environmental Microbiology·Peter Kamp Busk, Lene Lange
Aug 9, 2011·Applied and Environmental Microbiology·James A LangstonMatt D Sweeney
Jan 1, 2011·Annual Review of Chemical and Biomolecular Engineering·Shishir P S ChundawatBruce E Dale
Feb 25, 2010·Biotechnology for Biofuels·Valdeir Arantes, Jack N Saddler
Jul 4, 2012·Biotechnology for Biofuels·Svein Jarle HornVincent Gh Eijsink
Dec 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Seonah KimGregg T Beckham
Apr 16, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jane W AggerBjørge Westereng
Sep 10, 2014·Proceedings of the National Academy of Sciences of the United States of America·Van V VuMichael A Marletta
Aug 19, 2014·Applied Microbiology and Biotechnology·In Jung KimKyoung Heon Kim
Dec 24, 2013·Nature Chemical Biology·Glyn R HemsworthPaul H Walton
Oct 20, 2015·Applied Microbiology and Biotechnology·Thamy Lívia Ribeiro CorrêaGonçalo Amarante Guimarães Pereira
Jul 26, 2012·Biotechnology Journal·Gernot Jäger, Jochen Büchs
Mar 19, 2015·Annual Review of Biochemistry·William T BeesonMichael A Marletta
Jan 1, 2012·Computational and Structural Biotechnology Journal·Leila Lo LeggioLeonardo De Maria
Nov 28, 2014·Microbiology and Molecular Biology Reviews : MMBR·Johanna RytiojaMiia R Mäkelä
Nov 28, 2014·Microbiology and Molecular Biology Reviews : MMBR·Fernando SegatoRolf A Prade
May 27, 2011·Trends in Biotechnology·Alexander V Gusakov
Jan 4, 2017·Acta Crystallographica. Section D, Structural Biology·Kristian E H FrandsenLeila Lo Leggio
Oct 18, 2016·The Analyst·Chris Jeynes, Julien L Colaux

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aspergillosis

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Cardiac Glycosides

Cardiac glycosides are a diverse family of naturally derived compounds that bind to and inhibit na+/k+-atpase. Discover the latest research on cardiac glycosides heres.

Related Papers

Protein Science : a Publication of the Protein Society
Zarah ForsbergVincent G H Eijsink
Proceedings of the National Academy of Sciences of the United States of America
R Jason QuinlanPaul H Walton
© 2022 Meta ULC. All rights reserved