The fission yeast Nup107-120 complex functionally interacts with the small GTPase Ran/Spi1 and is required for mRNA export, nuclear pore distribution, and proper cell division

Molecular and Cellular Biology
Siau Wei BaïValérie Doye

Abstract

We have characterized Schizosaccharomyces pombe open reading frames encoding potential orthologues of constituents of the evolutionarily conserved Saccharomyces cerevisiae Nup84 vertebrate Nup107-160 nuclear pore subcomplex, namely Nup133a, Nup133b, Nup120, Nup107, Nup85, and Seh1. In spite of rather weak sequence conservation, in vivo analyses demonstrated that these S. pombe proteins are localized at the nuclear envelope. Biochemical data confirmed the organization of these nucleoporins within conserved complexes. Although examination of the S. cerevisiae and S. pombe deletion mutants revealed different viability phenotypes, functional studies indicated that the involvement of this complex in nuclear pore distribution and mRNA export has been conserved between these highly divergent yeasts. Unexpectedly, microscopic analyses of some of the S. pombe mutants revealed cell division defects at the restrictive temperature (abnormal septa and mitotic spindles and chromosome missegregation) that were reminiscent of defects occurring in several S. pombe GTPase Ran (Ran(Sp))/Spi1 cycle mutants. Furthermore, deletion of nup120 moderately altered the nuclear location of Ran(Sp)/Spi1, whereas overexpression of a nonfunctional Ran(Sp)/Spi...Continue Reading

References

Jan 1, 1991·Methods in Enzymology·S MorenoP Nurse
Apr 1, 1966·The Journal of Cell Biology·C F Robinow, J Marak
Feb 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·L F PembertonG Blobel
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·J DemeterS Sazer
Jun 25, 1993·Nucleic Acids Research·S L Forsburg
Dec 1, 1995·The Journal of Cell Biology·J D AitchisonM P Rout
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Jun 3, 1997·Gene·S L Forsburg, D A Sherman
Jul 22, 1997·Proceedings of the National Academy of Sciences of the United States of America·X HeS Sazer
Dec 8, 1998·Molecular Biology of the Cell·N BelgarehV Doye
Nov 5, 1999·The Journal of Biological Chemistry·M T TeixeiraB Dujon
Feb 23, 2000·The Journal of Cell Biology·M P RoutB T Chait
Apr 4, 2000·The Journal of Cell Biology·S SiniossoglouE Hurt
Nov 22, 2000·The Journal of Cell Biology·U FleigS Sazer
Jan 9, 2001·Nature Cell Biology·K N ClouseR Reed
Apr 20, 2001·The Journal of Cell Biology·P T TranF Chang
Oct 31, 2001·The Journal of Cell Biology·S VasuD J Forbes
Dec 1, 2001·Microbiology and Molecular Biology Reviews : MMBR·I G Macara
Aug 15, 2002·Current Biology : CB·Mary Dasso
Aug 28, 2002·The Journal of Cell Biology·Janet M CronshawMichael J Matunis

❮ Previous
Next ❯

Citations

Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hyuk-Soo SeoAndré Hoelz
Aug 14, 2009·The Journal of Biological Chemistry·James R R Whittle, Thomas U Schwartz
Jun 30, 2006·Molecular Biology of the Cell·Arturo V OrjaloDouglass J Forbes
Sep 22, 2006·Molecular Biology of the Cell·Aysha H OsmaniStephen A Osmani
Jan 4, 2008·Molecular Biology of the Cell·Ulrike TheisenGero Steinberg
Jun 20, 2008·Molecular Biology of the Cell·Katerina R KatsaniValérie Doye
Jul 31, 2009·Molecular Biology of the Cell·Corine K LauDouglass J Forbes
Mar 21, 2012·Genes & Development·Katrina J WoolcockMarc Bühler
Jan 4, 2008·Molecular and Cellular Biology·Karen K ResendesDouglass J Forbes
Oct 11, 2005·Annual Review of Cell and Developmental Biology·Martin W HetzerIain W Mattaj
Apr 19, 2011·Annual Review of Biochemistry·André HoelzGünter Blobel
Apr 8, 2010·Cellular and Molecular Life Sciences : CMLS·Richard WozniakValérie Doye
Nov 14, 2007·The Journal of Cell Biology·Frank R Neumann, Paul Nurse
Feb 13, 2016·Frontiers in Cell and Developmental Biology·Haruhiko AsakawaTokuko Haraguchi
Jul 3, 2015·FEBS Open Bio·Haruhiko AsakawaTokuko Haraguchi
Oct 21, 2015·The Journal of Cell Biology·Hui-Ju YangYasushi Hiraoka
Sep 13, 2008·Trends in Cell Biology·Maximiliano A D'Angelo, Martin W Hetzer
Apr 10, 2013·Current Opinion in Cell Biology·Lucas BukataMaximiliano A D'Angelo
Feb 1, 2012·The Plant Journal : for Cell and Molecular Biology·Marcel WiermerXin Li
May 13, 2015·The Journal of Cell Biology·Rafael LucenaJuan Jimenez
Jun 21, 2012·Nucleus·Cristina González-Aguilera, Peter Askjaer
Dec 30, 2008·Molecular Cell·Erik W DeblerAndré Hoelz
Dec 28, 2007·Cell·Kuo-Chiang HsiaAndré Hoelz
Jan 14, 2012·Biochemical and Biophysical Research Communications·Nobuyoshi WatanabeTokio Tani
May 24, 2019·PLoS Genetics·Guillaume Holzer, Wolfram Antonin
Feb 3, 2005·Yeast
Feb 11, 2005·Eukaryotic Cell·B Booth QuimbyMary Dasso
May 28, 2019·Cellular and Molecular Life Sciences : CMLS·Silvia Salas-Pino, Rafael R Daga

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.