PMID: 9531532May 9, 1998Paper

The fission yeast SPB component Cut12 links bipolar spindle formation to mitotic control

Genes & Development
Alan J BridgeIain M Hagan

Abstract

During fission yeast mitosis, the duplicated spindle pole bodies (SPBs) nucleate microtubule arrays that interdigitate to form the mitotic spindle. cut12.1 mutants form a monopolar mitotic spindle, chromosome segregation fails, and the mutant undergoes a lethal cytokinesis. The cut12(+) gene encodes a novel 62-kD protein with two predicted coiled coil regions, and one consensus phosphorylation site for p34(cdc2) and two for MAP kinase. Cut12 is localized to the SPB throughout the cell cycle, predominantly around the inner face of the interphase SPB, adjacent to the nucleus. cut12(+) is allelic to stf1(+); stf1.1 is a gain-of-function mutation bypassing the requirement for the Cdc25 tyrosine phosphatase, which normally dephosphorylates and activates the p34(cdc2)/cyclin B kinase to promote the onset of mitosis. Expressing a cut12(+) cDNA carrying the stf1.1 mutation also suppressed cdc25.22. The spindle defect in cut12.1 is exacerbated by the cdc25.22 mutation, and stf1.1 cells formed defective spindles in a cdc25.22 background at high temperatures. We propose that Cut12 may be a regulator or substrate of the p34(cdc2) mitotic kinase.

References

Aug 1, 1992·Molecular Biology of the Cell·T IzumiJ L Maller
Jan 1, 1991·Cold Spring Harbor Symposia on Quantitative Biology·J D HudsonP G Young
Feb 28, 1991·Nature·C Featherstone, P Russell
May 24, 1991·Science·A LupasJ Stock
Feb 28, 1990·Biochemical and Biophysical Research Communications·B DucommunD Beach
May 9, 1986·Cell·M Kirschner, T Mitchison
Apr 1, 1983·The Journal of Cell Biology·B R Oakley, N R Morris
Apr 1, 1995·Molecular Biology of the Cell·G J Den HaeseK L Gould
Jan 1, 1995·Molecular Biology of the Cell·P R MuellerW G Dunphy
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·J DemeterS Sazer
Feb 1, 1994·Molecular Biology of the Cell·J KuangJ E Penkala
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·J M WestendorfL Gerace
Jan 1, 1993·Molecular & General Genetics : MGG·S A MacNeill, P Nurse

❮ Previous
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Citations

Feb 27, 1999·Current Opinion in Cell Biology·W S Saunders
Apr 21, 1999·Current Opinion in Cell Biology·R Ohi, K L Gould
Dec 12, 2012·Nature Cell Biology·Agnes GrallertIain M Hagan
Jan 26, 2002·Molecular Biology of the Cell·Satoshi KatayamaTakashi Toda
Sep 13, 2001·Molecular Biology of the Cell·B KniolaK Ekwall
Jul 15, 2003·Molecular Biology of the Cell·Anne PaolettiMichel Bornens
Oct 21, 2003·Molecular Biology of the Cell·Vladimir S NekrasovJohn V Kilmartin
Oct 29, 2010·Molecular Biology of the Cell·Wen-zhu LiQuan-wen Jin
Apr 3, 2002·The EMBO Journal·Michael J E KrienMatthew J O'Connell
May 18, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Mitsuhiro Yanagida
Mar 6, 2003·Genome Research·Anabelle DecottigniesPaul Nurse
Jun 2, 2006·Molecular and Cellular Biology·Thein Z WinShao-Win Wang
Jul 5, 2001·Molecular and Cellular Biology·C R BartholomewC F Hardy
Sep 21, 2002·Molecular and Cellular Biology·Quan-Wen JinUrsula Fleig
Oct 23, 2010·PLoS Biology·Stefania CastagnettiPaul Nurse
May 11, 2000·Proceedings of the National Academy of Sciences of the United States of America·L Chang, K L Gould
Sep 22, 2006·Current Genetics·Stéphanie La Carbona, Xavier Le Goff
Aug 12, 2014·Molecular Biology of the Cell·Dan Zhang, Snezhana Oliferenko
Jul 23, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Keisuke IshiharaTimothy J Mitchison
Jul 23, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Christian ArquintErich A Nigg
Feb 22, 2013·Molecular Biology of the Cell·Ngang Heok TangTakashi Toda
Nov 22, 2000·The Journal of Cell Biology·U FleigS Sazer
Apr 24, 2002·The Journal of Cell Biology·Gislene PereiraElmar Schiebel

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