The fission yeast Stn1-Ten1 complex limits telomerase activity via its SUMO-interacting motif and promotes telomeres replication

Science Advances
Samah MatmatiStéphane Coulon

Abstract

Mammalian CST (CTC1-STN1-TEN1) complex fulfills numerous functions including rescue of the stalled replication forks and termination of telomerase action. In fission yeast lacking the CTC1 ortholog, the Stn1-Ten1 complex restricts telomerase action via its sumoylation-mediated interaction with Tpz1TPP1. We identify a small ubiquitin-like modifier (SUMO)-interacting motif (SIM) in the carboxyl-terminal part of Stn1 and show that this domain is crucial for SUMO and Tpz1-SUMO interactions. Point mutations in the SIM (Stn1-226) lead to telomere elongation, impair Stn1-Ten1 recruitment to telomeres, and enhance telomerase binding, revealing that Stn1 SIM domain contributes to the inhibition of telomerase activity at chromosome ends. Our results suggest that Stn1-Ten1 promotes DNA synthesis at telomeres to limit single-strand DNA accumulation. We further demonstrate that Stn1 functions in the replication of telomeric and subtelomeric regions in a Taz1-independent manner. Genetic analysis reveals that misregulation of origin firing and/or telomerase inhibition circumvents the replication defects of the stn1-226 mutant. Together, our results show that the Stn1-Ten1 complex has a dual function at telomeres by limiting telomerase action ...Continue Reading

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Citations

Apr 13, 2019·Nucleic Acids Research·Miaomiao ZhangFeng Wang
Feb 24, 2019·The EMBO Journal·Jose Miguel EscandellMiguel Godinho Ferreira
Sep 3, 2020·Molecular Biology of the Cell·Victoria A HassebroekYoshiaki Azuma
Mar 22, 2020·Current Opinion in Genetics & Development·Erin Henninger, Maria T Teixeira
Apr 20, 2021·Frontiers in Cell and Developmental Biology·Erin BonnellRaymund J Wellinger

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Methods Mentioned

BETA
two-hybrid
Y2H
coimmunoprecipitation
co-IP
pulldown
dot blot
dissection
electrophoresis
dissections
immunoprecipitation

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