The five glucose-6-phosphatase paralogous genes are differentially regulated by insulin alone or combined with high level of amino acids and/or glucose in trout hepatocytes

Molecular Biology Reports
Marandel LucieSkiba-Cassy Sandrine

Abstract

A recent analysis of the newly sequenced rainbow trout (Oncorhynchus mykiss) genome suggested that duplicated gluconeogenic g6pc paralogues, fixed in this genome after the salmonid-specific 4th whole genome duplication, may have a role in the setting up of the glucose-intolerant phenotype in this carnivorous species. This should be due to the sub- or neo-functionalization of their regulation. In the present short communication we thus addressed the question of the regulation of these genes by insulin, hormone involved in the glucose homeostasis, and its interaction with glucose and amino acids in vitro. The stimulation of trout hepatocytes with insulin revealed an atypical up-regulation of g6pcb2 ohnologues and confirmed the sub- or neo-functionalization of the five g6pc genes at least at the regulatory level. Intriguingly, when hepatocytes were cultured with high levels of glucose and/or AAs in presence of insulin, most of the g6pc paralogues were up-regulated. It strongly suggested a cross-talk between insulin and nutrients for the regulation of these genes. Moreover these results strengthened the idea that g6pc duplicated genes may significantly contribute to the setting up of the glucose-intolerant phenotype in trout via th...Continue Reading

References

Aug 11, 2001·Biochemical Society Transactions·R M O'BrienL A Hornbuckle
Jul 20, 2002·Aquatic Toxicology·Michela FerrarisEnzo Chiesara
Jan 28, 2003·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·S KirchnerS Panserat
Oct 12, 2004·General and Comparative Endocrinology·Maria del sol NovoaIsabel Navarro
Jan 9, 2008·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Séverine KirchnerRonaldo P Ferraris
Oct 2, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·S PolakofS Panserat
Nov 12, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·S PolakofS Panserat
Jan 7, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Iban SeiliezSandrine Skiba-Cassy
Aug 29, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Weiwei DaiSandrine Skiba-Cassy
Jun 27, 2015·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Weiwei DaiSandrine Skiba-Cassy

❮ Previous
Next ❯

Citations

Nov 13, 2018·Annual Review of Animal Biosciences·S PanseratS Skiba-Cassy
Feb 9, 2017·BMC Evolutionary Biology·Nasser M Al-DaghriManuela Sironi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.