The flagellin N-methylase gene fliB and an adjacent serovar-specific IS200 element in Salmonella typhimurium.

Microbiology
André P BurnensJacques Nicolet

Abstract

The cloning and molecular genetic analysis of a locus mapping within the flagellar gene (fli) complex of Salmonella typhimurium is reported. A copy of the insertion element IS200 was located in a noncoding stretch of DNA upstream of the fliA gene. Comparative nucleotide sequence analysis showed that this copy of IS200 was 711 bp long and that its flanking regions contained no features common to other characterized insertion sites of this element. The element was located 37 bp downstream of an ORF whose product was shown by interspecific transfer and amino acid analysis to carry out N-methylation of selected lysine residues in Salmonella flagellin. The sequence and phenotype of this ORF identified it as fliB, encoding the only prokaryotic N-methylase acting on amino groups to have been characterized to date. It was found to be conserved among all clinically significant serovars of Salmonella. The IS200 insertion site is of particular interest since it was conserved in all but two rare evolutionary lines of S. typhimurium, and was absent from 85 Salmonella strains belonging to 37 other serovars. It is thus a phylogenetically significant marker at the serovar level.

References

Dec 14, 1979·Biochimica Et Biophysica Acta·T M Joys, H Kim
Mar 1, 1976·Journal of General Microbiology·R KonnoS Yamaguchi
Mar 25, 1991·Nucleic Acids Research·I GibertJ Casadesus
Mar 1, 1991·Journal of General Microbiology·P BeltranR K Selander
May 1, 1990·Journal of Bacteriology·N H SmithR K Selander
Apr 1, 1989·Molecular & General Genetics : MGG·J Casadesus, J R Roth
Oct 1, 1989·Molecular Microbiology·G FrankelB A Stocker
Dec 1, 1985·Journal of Clinical Microbiology·G F IbrahimR A Walker
Oct 1, 1983·Cell·S Lam, J R Roth
May 1, 1995·Journal of Clinical Microbiology·J StanleyA Burnens
May 1, 1995·Journal of Bacteriology·M IriarteG R Cornelis
Aug 1, 1994·Journal of Medical Microbiology·J StanleyA P Burnens
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M S StromS Lory
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·J LiR K Selander
Dec 1, 1993·Journal of Bacteriology·K E SandersonA Hessel
Oct 1, 1993·Journal of General Microbiology·E EzquerraJ Stanley

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Citations

Sep 6, 2006·Nature Reviews. Microbiology·Mark J Pallen, Nicholas J Matzke
Sep 3, 1999·Nucleic Acids Research·C R BeuzónJ Casadesús
Jun 9, 2004·Journal of Clinical Microbiology·Silvia Herrera-LeónM Aurora Echeita
Sep 19, 2006·FEMS Microbiology Letters·Susana MerinoJuan M Tomás
Sep 16, 2005·The EMBO Journal·Bao Ton-HoangMichael Chandler
Sep 28, 1998·Journal of Bacteriology·G LeclercB Ely
Jan 1, 2006·EcoSal Plus·Elise R Hondorp, Rowena G Matthews
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·J Mahillon, M Chandler

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