The flavonoid kurarinone inhibits clinical progression of EAE through inhibiting Th1 and Th17 cell differentiation and proliferation

International Immunopharmacology
Liang XieWei Wu

Abstract

The flavonoid kurarinone suppresses CD4+ T-cell-mediated chronic inflammatory dermatitis. However, kurarinone's effects upon autoimmune central nervous system (CNS) disease remain unknown. We investigated the potential therapeutic effects and molecular mechanism(s) of kurarinone in an experimental autoimmune encephalomyelitis (EAE) murine model of multiple sclerosis (MS). Myelin oligodendrocyte glycoprotein (MOG35-55) peptide-induced EAE was constructed in wild-type mice. Effects of kurarinone (100 mg/kg/day) upon clinical scores were assessed based on physical traits and signs. Spinal cord sections were extracted to assess inflammation, demyelination, and mRNA expression of key pro-inflammatory cytokines and chemokines. CNS-infiltrating mononuclear cells (MNCs) and splenocytes were harvested; flow cytometry was then applied to determine CD4+ and CD8+ T-cell percentages as well as Th1/Th2/Th17 subset percentages. Purified naïve CD4+ T-cells underwent in vitro T-cell polarization and proliferation to assess kurarinone's effects. Prophylactic and treatment regimens of kurarinone significantly improved clinical scores in the MOG35-55 peptide-induced EAE model (P < 0.05). Kurarinone significantly lowered CNS inflammation and demyel...Continue Reading

Citations

Jul 14, 2020·Biomedical Chromatography : BMC·Yiqian HuangXiaosong Huang
Oct 3, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Youfa QinJian Wang
Oct 22, 2020·Acta Biochimica Et Biophysica Sinica·Li ZhangJun Xie
May 1, 2021·International Journal of Molecular Sciences·Kuo-Tung TangSen-Wei Tsai
Aug 4, 2019·Journal of Neuroimmunology·Lucian Del FabbroCristiano Ricardo Jesse
Aug 28, 2021·International Journal of Molecular Sciences·Soo-Jin ParkHyun-Jun Lee

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