The flavoproteins CryD and VvdA cooperate with the white collar protein WcoA in the control of photocarotenogenesis in Fusarium fujikuroi

PloS One
Marta Castrillo, Javier Avalos

Abstract

Light stimulates carotenoid biosynthesis in the ascomycete fungus Fusarium fujikuroi through transcriptional activation of the structural genes of the pathway carRA, carB, and cart, but the molecular basis of this photoresponse is unknown. The F. fujikuroi genome contains genes for different predicted photoreceptors, including the WC protein WcoA, the DASH cryptochrome CryD and the Vivid-like flavoprotein VvdA. We formerly found that null mutants of wcoA, cryD or vvdA exhibit carotenoid photoinduction under continuous illumination. Here we show that the wild type exhibits a biphasic response in light induction kinetics experiments, with a rapid increase in carotenoid content in the first hours, a transient arrest and a subsequent slower increase. The mutants of the three photoreceptors show different kinetic responses: the wcoA mutants are defective in the rapid response, the cryD mutants are affected in the slower response, while the fast and slow responses were respectively enhanced and attenuated in the vvdA mutants. Transcriptional analyses of the car genes revealed a strong reduction of dark and light-induced transcript levels in the wcoA mutants, while minor or no reductions were found in the cryD mutants. Formerly, we fo...Continue Reading

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Citations

Sep 17, 2015·Environmental Microbiology·Arko DasguptaJennifer J Loros
Jul 29, 2015·Photochemistry and Photobiology·Marta CastrilloRichard Pokorny
Jun 27, 2017·Fungal Genetics and Biology : FG & B·Julia Schumacher
Mar 20, 2020·Bioscience, Biotechnology, and Biochemistry·Khanh Dung PhamWataru Ogasawara
Jul 15, 2020·Biological Chemistry·Stephan KiontkeAlfred Batschauer
Jan 27, 2018·Journal of Fungi·Javier AvalosMaría Carmen Limón
Jan 22, 2020·Scientific Reports·Obdulia Parra-RiveroJavier Avalos
Feb 13, 2021·Frontiers in Microbiology·Javier Pardo-MedinaJavier Avalos
Mar 16, 2021·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Aba Losi, Wolfgang Gärtner
May 12, 2021·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Xin GeQi Xin
Sep 19, 2020·Current Biology : CB·Eusebio NavarroVictoriano Garre
Jun 15, 2021·Bioscience, Biotechnology, and Biochemistry·Khanh Dung PhamWataru Ogasawara

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Datasets Mentioned

BETA
HE650104
CCT69959
X90699

Methods Mentioned

BETA
PCR
phosphotransferase
electrophoresis

Software Mentioned

GraphPad Prism

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