The FLC dimer with lambda type may false-migrate to the position of "albumin" band by urine protein electrophoresis on Sebia agarose gel-based detection system

Journal of Clinical Laboratory Analysis
Changqiang ChenXuqian Fang

Abstract

Monoclonal free light chains (FLC) commonly exist in monomeric or dimeric forms but rarely as larger molecules. Little is known about whether polymeric molecules can affect urine protein electrophoresis (UPE) results. Urine samples were collected from 72 multiple myeloma (MM) patients with Bence Jones protein (BJP). Urine protein and immunofixation electrophoresis were analyzed on Sebia SDS "agarose" gel electrophoresis system (SDS-AGE), and immunoglobulin free light chains were measured on the BNII nephelometric assay. A type of disulfide-bound FLC dimer shows a pattern shift to the position of the "albumin" band in urine protein electrophoresis in multiple myeloma (MM) patients according to the Sebia agarose gel-based detection system, which was validated by immunofixation, SDS-PAGE, and mass spectrometric methods. Similar cases were found in 21 (29.17%) of 72 MM patients with BJP, and 19 (90.5%) of 21 patients were the lambda type. These results indicate that BJP with lambda type has a strong tendency to abnormally migrate, which may increase the risk of misinterpretation of protein electrophoresis in clinics. Thus, when the urine protein electrophoresis is inconsistent with the result by nephelometric method, urine protein ...Continue Reading

References

Sep 1, 1976·Scandinavian Journal of Clinical and Laboratory Investigation·K Sölling
Jul 15, 1974·Clinica Chimica Acta; International Journal of Clinical Chemistry·L BalantJ Fabre
Jan 1, 1984·Acta Medica Scandinavica·K SøllingJ Lanng Nielsen
Nov 22, 2000·Pharmaceutical Research·M PerkinsM Jeschke
Feb 13, 2008·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·Laura A Sikkink, Marina Ramirez-Alvarado
Jun 14, 2008·American Journal of Clinical Pathology·Anja C RodenJerry A Katzmann
Sep 28, 2010·Journal of Neuroimmunology·Batia KaplanBen-Ami Sela
Mar 15, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·Takanari NakanoTakayuki Murakoshi
Mar 29, 2011·TheScientificWorldJournal·Batia KaplanBen-Ami Sela

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Jan 1, 2021·Talanta·Deepak KumarDibyajyoti Banerjee

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