The fluorometric microculture cytotoxicity assay

Nature Protocols
Elin LindhagenRolf Larsson

Abstract

The fluorometric microculture cytotoxicity assay (FMCA) is a nonclonogenic microplate-based cell viability assay used for measurement of the cytotoxic and/or cytostatic effect of different compounds in vitro. The assay is based on hydrolysis of the probe, fluorescein diacetate (FDA) by esterases in cells with intact plasma membranes. The assay is available as both a semiautomated 96-well plate setup and a 384-well plate version fully adaptable to robotics. Experimental plates are prepared with a small amount of drug solution and can be stored frozen. Cells are seeded on the plates and cell viability is evaluated after 72 h. The protocol described here is applicable both for cell lines and freshly prepared tumor cells from patients and is suitable both for screening in drug development and as a basis for a predictive test for individualization of anticancer drug therapy.

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Citations

Oct 19, 2013·Journal of Cancer Research and Clinical Oncology·Peter NygrenRolf Larsson
Nov 28, 2012·Investigational New Drugs·Jenny FelthLinda Rickardson
Oct 1, 2013·Phytochemistry·Tomohiro MinakawaMasami Ishibashi
Jul 9, 2013·BMC Cancer·Sara StreseJoachim Gullbo
Jan 20, 2011·PloS One·Helene HallböökJoachim Gullbo
May 30, 2012·Mutation Research·Marimawit Y YeshakBjörn Hellman
Jul 22, 2015·Annals of Surgical Oncology·Kathrine BjersandPeter Nygren
Dec 17, 2015·The Journal of Antibiotics·Mohamed S AbdelfattahMasami Ishibashi
Oct 30, 2014·Chemical Biology & Drug Design·Iten M FawzyKhaled A M Abouzid
Sep 6, 2012·International Journal of Cancer. Journal International Du Cancer·Malin WickströmJohn I Johnsen
Sep 14, 2011·Cancer Science·Saadia HassanPeter Nygren
Apr 10, 2015·Chemical Biology & Drug Design·Xin WangStig Linder
Jun 27, 2014·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Meng-Yin YangHsin-I Ma
May 28, 2015·Journal of Natural Medicines·Rolly G FuentesMasami Ishibashi
Jun 1, 2015·Journal of Natural Medicines·Midori A AraiMasami Ishibashi
May 30, 2015·Medical Oncology·Thomas StrömbergGunilla Enblad
Jul 27, 2011·Biochimica Et Biophysica Acta·Robert BurmanUlf Göransson
Dec 4, 2012·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Franzisca Marie BätzMonika Schäfer-Korting
Feb 14, 2014·Leukemia Research·Daniel J DeangeloRichard M Stone
Jan 16, 2013·Biochemical and Biophysical Research Communications·Malin JarviusRolf Larsson

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