PMID: 9173869Apr 1, 1997Paper

The flux control coefficient of carnitine palmitoyltransferase I on palmitate beta-oxidation in rat hepatocyte cultures

The Biochemical Journal
T D SpurwayL Agius

Abstract

Two important factors that determine the flux of hepatic beta-oxidation of long-chain fatty acids are the availability of fatty acid and the activity of carnitine palmitoyltransferase I (CPT I). Using Metabolic Control Analysis, the flux control coefficient of CPT I in rat hepatocyte monolayers was determined by titration with 2-[6-(4-chlorophenoxy)hexyl]oxirane-2-carboxylate (Etomoxir), which is converted to Etomoxir-CoA, an irreversible inhibitor of CPT I. We measured CPT I activity and flux through beta-oxidation at 0.2 mM and 1.0 mM palmitate to simulate substrate concentrations in fed and fasted states. Rates of beta-oxidation were 4.5-fold higher at 1. 0 mM palmitate compared with 0.2 mM palmitate. Flux control coefficients of CPT I, estimated by two independent methods, were similar: 0.67 and 0.79 for 0.2 mM palmitate, and 0.68 and 0.77 for 1 mM palmitate. It is concluded that the regulatory potential of CPT I is similar at low and high physiological concentrations of palmitate.

Citations

Jun 19, 2009·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·R K SuarezL G Herrera M
Apr 4, 2017·PLoS Computational Biology·Anne-Claire M F MartinesBarbara M Bakker
Oct 6, 2011·The Journal of Biological Chemistry·Lijun LiuMarcus P Cooper
Mar 31, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Priska KaufmannStephan Krähenbühl
Apr 21, 2007·Cardiovascular Drug Reviews·Houman AshrafianMichael P Frenneaux
Dec 15, 2010·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Edwin R PriceChristopher G Guglielmo
Jan 15, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Jennifer Y Kong, Simon W Rabkin
Feb 18, 2004·Chemical Research in Toxicology·Russell J Mortishire-SmithJeremy K Nicholson
Jul 11, 2001·Biochemical and Biophysical Research Communications·S EatonP A Quant
Jan 30, 2002·Progress in Lipid Research·Simon Eaton

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