The Focal Adhesion Analysis Server: a web tool for analyzing focal adhesion dynamics

F1000Research
Matthew E Berginski, Shawn M Gomez

Abstract

The Focal Adhesion Analysis Server (FAAS) is a web-based implementation of a set of computer vision algorithms designed to quantify the behavior of focal adhesions in cells imaged in 2D cultures. The input consists of one or more images of a labeled focal adhesion protein. The outputs of the system include a range of static and dynamic measurements for the adhesions present in each image as well as how these properties change over time. The user is able to adjust several parameters important for proper focal adhesion identification. This system provides a straightforward tool for the global, unbiased assessment of focal adhesion behavior common in optical microscopy studies. The webserver is available at: http://faas.bme.unc.edu/.

References

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Methods Mentioned

BETA
fluorescence microscopy

Software Mentioned

NIH ImageJ
Focal Adhesion Analysis Server

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