The foldon substructure of staphylococcal nuclease.

Journal of Molecular Biology
Sabrina BédardS W Englander

Abstract

To search for submolecular foldon units, the spontaneous reversible unfolding and refolding of staphylococcal nuclease under native conditions was studied by a kinetic native-state hydrogen exchange (HX) method. As for other proteins, it appears that staphylococcal nuclease is designed as an assembly of well-integrated foldon units that may define steps in its folding pathway and may regulate some other functional properties. The HX results identify 34 amide hydrogens that exchange with solvent hydrogens under native conditions by way of large transient unfolding reactions. The HX data for each hydrogen measure the equilibrium stability (Delta G(HX)) and the kinetic unfolding and refolding rates (k(op) and k(cl)) of the unfolding reaction that exposes it to exchange. These parameters separate the 34 identified residues into three distinct HX groupings. Two correspond to clearly defined structural units in the native protein, termed the blue and red foldons. The remaining HX grouping contains residues, not well separated by their HX parameters alone, that represent two other distinct structural units in the native protein, termed the green and yellow foldons. Among these four sets, a last unfolding foldon (blue) unfolds with a r...Continue Reading

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Citations

Apr 15, 2008·Quarterly Reviews of Biophysics·S Walter EnglanderMallela M G Krishna
May 16, 2008·Proceedings of the National Academy of Sciences of the United States of America·Sabrina BédardS Walter Englander
Sep 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ying WuOsman Bilsel
Jun 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Rachel BernsteinSusan Marqusee
Apr 13, 2012·Proceedings of the National Academy of Sciences of the United States of America·Julien RocheCatherine A Royer
Sep 11, 2013·Proceedings of the National Academy of Sciences of the United States of America·Zhong-Yuan KanS Walter Englander
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