The force-sensitive protein Ajuba regulates cell adhesion during epithelial morphogenesis

The Journal of Cell Biology
William RazzellJennifer A Zallen

Abstract

The reorganization of cells in response to mechanical forces converts simple epithelial sheets into complex tissues of various shapes and dimensions. Epithelial integrity is maintained throughout tissue remodeling, but the mechanisms that regulate dynamic changes in cell adhesion under tension are not well understood. In Drosophila melanogaster, planar polarized actomyosin forces direct spatially organized cell rearrangements that elongate the body axis. We show that the LIM-domain protein Ajuba is recruited to adherens junctions in a tension-dependent fashion during axis elongation. Ajuba localizes to sites of myosin accumulation at adherens junctions within seconds, and the force-sensitive localization of Ajuba requires its N-terminal domain and two of its three LIM domains. We demonstrate that Ajuba stabilizes adherens junctions in regions of high tension during axis elongation, and that Ajuba activity is required to maintain cell adhesion during cell rearrangement and epithelial closure. These results demonstrate that Ajuba plays an essential role in regulating cell adhesion in response to mechanical forces generated by epithelial morphogenesis.

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Citations

Mar 7, 2019·Journal of Cell Science·Cordelia RauskolbKenneth D Irvine
May 10, 2019·Cell Cycle·Tara M Finegan, Dan T Bergstralh
Jan 10, 2020·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Jia GouHans G Othmer
Jul 23, 2019·F1000Research·Willem-Jan PannekoekMartijn Gloerich
Aug 22, 2020·PLoS Computational Biology·Tomer SternEric F Wieschaus
Jan 12, 2021·Frontiers in Cell and Developmental Biology·Deqing Kong, Jörg Großhans
Sep 9, 2020·Current Opinion in Cell Biology·Priti Agarwal, Ronen Zaidel-Bar
Feb 10, 2021·Journal of Cell Science·Srividya VenkatramananKenneth D Irvine
Mar 26, 2020·Current Biology : CB·Floris Bosveld, Yohanns Bellaïche
May 25, 2021·Cytoskeleton·Caitlin A AndersonJonathan D Winkelman
Nov 12, 2021·The Journal of Cell Biology·Kia Z Perez-ValeMark Peifer

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BETA
GTPase
Transgenic
PCR

Software Mentioned

Zen
Volocity
GraphPad
EOS Utility
SIESTA
ImageJ
Prism

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