PMID: 42446Oct 24, 1979

The formation and thermal stability of in vitro assembled fibrils from acid-soluble and pepsin-treated collagens

Biochimica Et Biophysica Acta
J M Snowden, D A Swann

Abstract

The role of the non-helical regions of the collagen molecule in fibrillogenesis has been investigated by comparing the kinetics of fibril formation of pepsin-treated acid-soluble collagen, acid-soluble collagen and mixtures of the two and by comparison of the thermal stabilities of the fibrils formed. The acid-soluble collagen was found to aggregate more rapidly than the pepsin-treated collagen under physiological conditions of pH and ionic strength. Variations in ionic strength, at physiological pH, were found to have differing effects on the aggregation of these two forms of soluble collagen. Fibrils formed from the pepsinized-collagen had a lower thermal stability tha n those formed from the intact collagen. The behavior observed with mixtures of acid-soluble and pepsin-treated collagens was found to be quantitatively consistent with the pepsinized collagen being able to utilize the nuclei formed by the acid-soluble collagen for subsequent growth. However, the use of the acid-soluble nuclei by the pepsinized collagen for growth did not enhance its rate of precipitation during the growth phase, nor did it enhance the thermal stability of the fibrils formed from the pepsinized collagen.

References

Aug 1, 1989·Biomaterials·A K GargH G Garg
Aug 1, 1984·Collagen and Related Research·D E Birk, F H Silver
Aug 1, 1987·Collagen and Related Research·S AmudeswariB Chakrabarti
Apr 16, 2010·The Journal of Biological Chemistry·Saori KuniiBen'ichiro Tonomura
Oct 1, 1981·Biopolymers·M Brennan, P F Davison
May 23, 2006·Micron : the International Research and Review Journal for Microscopy·Ruggero TenniVittoria Ottani
Nov 15, 1984·Archives of Biochemistry and Biophysics·D E Birk, F H Silver
Jul 5, 2013·Biophysical Journal·Martijn de WildGijsje H Koenderink
Oct 19, 2012·Cell Adhesion & Migration·Peta BradburyGeraldine M O'Neill
Jan 13, 2015·Journal of Materials Science. Materials in Medicine·George W DombiSze C Yang
May 11, 2002·Journal of Biomechanical Engineering·Blayne A RoederSherry L Voytik-Harbin
Oct 1, 1980·Biopolymers·M Brennan, P F Davison

Citations

Oct 1, 1975·Archives of Biochemistry and Biophysics·T R OegemaD D Dziewiatkowski
Oct 1, 1977·Biopolymers·W D Comper, A Veis
Oct 1, 1977·Biopolymers·W D Comper, A Veis
Dec 1, 1968·Archives of Biochemistry and Biophysics·B P Toole, D A Lowther
Oct 1, 1973·The Journal of Clinical Investigation·A H Kang, R L Trelstad
Jan 1, 1966·Biochemistry·M P DrakeF O Schmitt
Jan 4, 1963·Science·A L RUBINF O Schmitt
Feb 1, 1960·Proceedings of the National Academy of Sciences of the United States of America·A J HodgeF O Schmitt
Feb 15, 1974·Science·H Y GöksuR Fryxell

Related Concepts

Aggregation
Pepsin 1
Zyderm
Soluble
Collagen Location
Pepsin A
Bos indicus
Hydrogen-Ion Concentration
Collagen Activity
Chemical Precipitation

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.