PMID: 8597574Feb 8, 1996Paper

The formation of a disulfide cross-link between the two subunits demonstrates the dimeric structure of the mitochondrial oxoglutarate carrier

Biochimica Et Biophysica Acta
F BisacciaFerdinando Palmieri

Abstract

Isolated oxoglutarate carrier (OGC) can be cross-linked to dimers by disulfide-forming reagents such as Cu2+-phenanthroline and diamide. Acetone and other solvents increase the extent of Cu2+ -phenanthroline-induced cross-linking of OGC. Cross-linked OGC re-incorporated in proteoliposomes fully retains the oxoglutarate transport activity. The amount of cross-linked OGC calculated by densitometry of scanned gels depends on the method of staining, since cross-linked OGC exhibits a higher sensitivity to Coomassie brilliant blue as compared to silver nitrate. Under optimal conditions the formation of cross-linked OGC dimer (stained with Coomassie brilliant blue) amounts to 75% of the total protein. Approximately the same cross-linking efficiency was evaluated from Western blots. Cross-linking of OGC is prevented by SH reagents and reversed by SH-reducing reagents, which shows that it is mediated by disulfide bridge(s). The formation of S-S bridge(s) requires the native state of the protein, since it is suppressed by SDS and by heating. Furthermore, the extent of cross-linking is independent of OGC concentration indicating that disulfide bridge(s) must be formed between the two subunits of native dimers. The number and localization ...Continue Reading

References

Jan 1, 1979·Methods in Enzymology·F Palmieri, M Klingenberg
Aug 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S Lindenthal, D Schubert
Jun 1, 1991·European Journal of Biochemistry·C IndiveriF Palmieri
Oct 24, 1990·Biochimica Et Biophysica Acta·C IndiveriF Palmieri
Mar 1, 1990·Research in Microbiology·P C Maloney
Mar 1, 1989·European Journal of Biochemistry·M Klingenberg, M Appel
Mar 4, 1987·Biochimica Et Biophysica Acta·C IndiveriR Krämer
Dec 16, 1985·Biochimica Et Biophysica Acta·F BisacciaF Palmieri
May 1, 1968·Biochimica Et Biophysica Acta·K Kobashi
Sep 25, 1972·European Journal of Biochemistry·F PalmieriM Klingenberger
Nov 6, 1969·Biochemical and Biophysical Research Communications·N S KosowerW S Correa
Mar 22, 1995·Biochimica Et Biophysica Acta·I StipaniF Palmieri
Jun 6, 1994·FEBS Letters·F Palmieri
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Pierre CardolGiovanni Finazzi
Jan 1, 1972·Methods in Enzymology·A F Habeeb

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Citations

Jun 22, 2002·Trends in Biochemical Sciences·Liesbeth M VeenhoffBert Poolman
Jul 3, 2009·Zhurnal evoliutsionnoĭ biokhimii i fiziologii·D A Aliverdieva, D V Mamaev
Jun 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Lisa BamberEdmund R S Kunji
Oct 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Lisa BamberEdmund R S Kunji
May 9, 2009·Biochimica Et Biophysica Acta·Steven M Claypool
Aug 13, 2005·Molecular Membrane Biology·Maria A Castiglione-MorelliFaustino Bisaccia
May 31, 2008·Biochimica Et Biophysica Acta·Martin Klingenberg
Apr 7, 2010·Biochimica Et Biophysica Acta·Edmund R S Kunji, Paul G Crichton
Oct 30, 2004·Molecular Membrane Biology·Maria A Castiglione-MorelliFaustino Bisaccia
Jul 27, 2010·Veterinary Pathology·L H Lash
Apr 28, 2004·FEBS Letters·Edmund R S Kunji
Oct 12, 2012·Journal of Bioenergetics and Biomembranes·Magnus MonnéGiuseppe Fiermonte
Aug 2, 2003·The Journal of Biological Chemistry·Edmund R S Kunji, Marilyn Harding
Apr 8, 1998·The American Journal of Physiology·T D ScholzB C Schutte
Aug 22, 2006·The Journal of Biological Chemistry·Yasuaki KabeHiroshi Handa
Jul 18, 1996·Biochimica Et Biophysica Acta·F PalmieriL Palmieri
Oct 28, 2008·Methods : a Companion to Methods in Enzymology·Edmund R S KunjiPearl Akamine

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