The formation of canine neutrophil extracellular traps induced by sodium arsenic in polymorphonuclear neutrophils

Chemosphere
Zhengkai WeiYunhe Fu

Abstract

As a novel defense effector mechanism of host innate immune system, neutrophil extracellular traps (NETs) plays an important role against pathogens infection and several diseases. In this study, we investigated the influence of sodium arsenic on novel effector mechanism of NETs generated by polymorphonuclear neutrophils (PMN) and the potential molecular mechanism. Sodium arsenic-induced NETs formation was observed by fluorescence confocal microscopy. Quantitation of NETs induced by sodium arsenic was determined by fluorescence microplate. In parallel experiments, inhibitors of ERK1/2-, p38 MAPK - signaling pathways were used. The results showed that sodium arsenic significantly induced formation of NETs-like structures in PMNs, and these extracellular thicker and thinner networks were mainly composed by DNA decorated with histones, neutrophils elastase (NE) and myeloperoxydase (MPO), which suggests main classical characteristics of NETs induced by sodium arsenic. Furthermore, arsenic markedly increased quantitation of NETs, which further confirmed that sodium arsenic indeed triggered NETs release. However, inhibition of NADPH oxidase, ERK1/2- and p38 MAPK-signaling pathways did not change sodium arsenic-induced NETs formation, ...Continue Reading

Citations

Feb 6, 2020·British Journal of Pharmacology·Yishan YeFlorent Malard
Aug 18, 2020·BioMed Research International·Shanshan RanShugang Li
Jan 10, 2021·Ecotoxicology and Environmental Safety·Aimin JiangZhengkai Wei
Aug 14, 2019·Ecotoxicology and Environmental Safety·Chaoqun WangZhengkai Wei
Dec 23, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Di WuZhengkai Wei
Oct 17, 2020·Human & Experimental Toxicology·Md Monirul Islam, Naoshi Takeyama
May 22, 2021·Toxicology and Applied Pharmacology·Ershun ZhouZhengtao Yang
Aug 17, 2021·Oxidative Medicine and Cellular Longevity·Yanyan SongHang Gao

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