The formation of electronically excited species in the human multiple myeloma cell suspension

Scientific Reports
Marek RácPavel Pospíšil

Abstract

In this study, evidence is provided on the formation of electronically excited species in human multiple myeloma cells U266 in the growth medium exposed to hydrogen peroxide (H2O2). Two-dimensional imaging of ultra-weak photon emission using highly sensitive charge coupled device camera revealed that the addition of H2O2 to cell suspension caused the formation of triplet excited carbonyls (3)(R = O)*. The kinetics of (3)(R = O)* formation in the real time, as measured by one-dimensional ultra-weak photon emission using low-noise photomultiplier, showed immediate enhancement followed by a slow decay. In parallel to the formation of (3)(R = O)*, the formation of singlet oxygen ((1)O2) in U266 cells caused by the addition of H2O2 was visualized by the imaging of (1)O2 using the green fluorescence of singlet oxygen sensor green detected by confocal laser scanning microscopy. Additionally, the formation of (1)O2 after the addition of H2O2 to cell suspension was detected by electron paramagnetic resonance spin-trapping spectroscopy using 2,2,6,6-tetramethyl-4-piperidone. Presented results indicate that the addition of H2O2 to cell suspension results in the formation of (3)(R = O)* and (1)O2 in U266 cell suspension. The contribution o...Continue Reading

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Citations

Oct 27, 2015·Journal of Photochemistry and Photobiology. B, Biology·Heta MattilaEsa Tyystjärvi
Jan 25, 2019·Luminescence : the Journal of Biological and Chemical Luminescence·Michal NowakDariusz Nowak
Oct 31, 2017·Oxidative Medicine and Cellular Longevity·Arnold N Onyango
Jul 28, 2019·PloS One·Martin DlaskMichal Cifra
Apr 13, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Michal NowakDariusz Nowak

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Methods Mentioned

BETA
electron paramagnetic resonance

Software Mentioned

- ASW
FV10

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