PMID: 6514380Dec 1, 1984Paper

The formation of surface membrane vesicles from schistosomula of Schistosoma mansoni

Parasitology
John R KuselM N Cordeiro

Abstract

Intact surface membrane vesicles were obtained and purified from the schistosomula of Schistosoma mansoni. The method of preparation of the vesicles involved treating the schistosomula in a buffered high salt medium for 1 h at 4 degrees C (Step 1), and then for 1 h at 25-37 degrees C (Step 2). Vesicles were formed from mechanically- and skin-transformed schistosomula, and their size and number depended on the temperature of Step 2. The majority of the vesicles expressed surface membrane concanavalin A receptors and parasite antigens on their outermost surfaces. When incubated with sensitized peripheral blood mononuclear cell preparations from humans exposed to schistosomal preparations, the vesicles stimulated lymphocyte transformation as effectively as soluble egg and adult worm antigens. Although both preparations contained identical proteins, small vesicles were less effective than large vesicles in stimulating lymphocyte transformation. Hence, vesicles prepared under a variety of conditions might be used to study those factors which influence the presentation of membrane-bound surface antigens to the immune system of the host.

References

Jan 1, 1977·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·Y NagaiJ Pellegrino
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Dec 28, 1973·Biochimica Et Biophysica Acta·G Poste, A C Allison
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Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L S BarakW W Webb

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Citations

May 1, 1990·International Journal for Parasitology·A N MacGregor, S J Shore
Jul 1, 1995·International Journal for Parasitology·P Sobhon, W Apinhasmit
Dec 1, 1995·Molecular and Biochemical Parasitology·G C Oliveira, W M Kemp
Sep 1, 1986·The Journal of Cell Biology·M FoleyI Moore

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