PMID: 9159200Nov 1, 1996Paper

The foundations of successful RT in situ PCR

Frontiers in Bioscience : a Journal and Virtual Library
G J Nuovo

Abstract

RT in situ PCR allows for the routine and rapid detection of low copy viral and human RNAs. Success with RT in situ PCR is best accomplished with formalin fixed, paraffin embedded material, which allows the study of archival material. The key variable for RT in situ PCR is protease digestion. The optimal digestion time, which is determined by testing a variety of protease digestion times, is defined by an intense signal in the nuclei of most cells irrespective of the primers used, and a loss of this signal with overnight digestion in DNase. This permits the target specific direct incorporation of the labeled nucleotide into the amplified cDNA. A lack of signal with the negative control (DNase, no RT) and an intense nuclear signal in most cells with the positive control (no DNase) is prerequisite for success with RT in situ PCR. The localization of the signal (cytoplasmic for human mRNAs and restricted to certain cell types) is another important indicator of successful RT in situ PCR. The one step rTth system allows for the reproducible amplification and detection of low copy RNA targets within a few hours. Matrix metalloprotease (MMPs) and their inhibitors (TIMPs) in cervical cancer are used as a model system for RT in situ PCR...Continue Reading

Citations

May 24, 2007·Journal of Veterinary Science·Luciana MandrioliGiuliano Bettini
Oct 27, 2001·Applied and Environmental Microbiology·T HoshinoY Inamori
Aug 28, 2004·Histopathology·F LewisA M Hanby
Aug 16, 2005·Archives of Oral Biology·Daisuke EkuniEdward E Putnins

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