The Fragment HMGA2-sh-3p20 from HMGA2 mRNA 3'UTR Promotes the Growth of Hepatoma Cells by Upregulating HMGA2

Scientific Reports
Yuan WangXiaodong Zhang

Abstract

High mobility group A2 (HMGA2) plays a crucial role in the development of cancer. However, the mechanism by which HMGA2 promotes the growth of hepatocellular carcinoma (HCC) remains unclear. Here, we explore the hypothesis that HMGA2 may enhance the growth of hepatoma cells through a fragment based on the secondary structure of HMGA2 mRNA 3'-untranslated region (3'UTR). Bioinformatics analysis showed that HMGA2 mRNA displayed a hairpin structure within its 3'UTR, termed HMGA2-sh. Mechanistically, RNA immunoprecipitation assays showed that the microprocessor Drosha or DGCR8 interacted with HMGA2 mRNA in hepatoma cells. Then, Dicer contributes to the generation of the fragment HMGA2-sh-3p20 from the HMGA2-sh. HMGA2-sh-3p20 was screened by PCR analysis. Interestingly, HMGA2-sh-3p20 increased the expression of HMGA2 through antagonizing the tristetraprolin (TTP)-mediated degradation of HMGA2. HMGA2-sh-3p20 inhibited the expression of PTEN by targeting the 3'UTR of PTEN mRNA. In addition, the overexpression of PTEN could downregulate HMGA2 expression. Significantly, we documented the ability of HMGA2-sh-3p20 to promote the growth of hepatoma cells in vitro and in vivo. Thus, we conclude that the fragment HMGA2-sh-3p20 from HMGA2 mRN...Continue Reading

References

Nov 9, 2004·Nature·Richard I GregoryRamin Shiekhattar
Dec 22, 2004·Molecular Cell·Bryan R Cullen
Sep 21, 2005·Journal of Medical Virology·Xiaodong ZhangLihong Ye
Jan 5, 2006·Nucleic Acids Research·Carine BarreauH Beverley Osborne
Jul 18, 2006·Nucleic Acids Research·Jan Krüger, Marc Rehmsmeier
Jun 30, 2007·Proceedings of the National Academy of Sciences of the United States of America·Scott ShellMarcus E Peter
Nov 16, 2007·Nature Reviews. Cancer·Alfredo Fusco, Monica Fedele
Dec 17, 2008·Cell·Scott M Hammond, Norman E Sharpless
Dec 17, 2009·The Journal of Biological Chemistry·Kazi Mokim AhmedWen-Hwa Lee
Apr 23, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Feng MaXuetao Cao
Nov 3, 2010·Nucleic Acids Research·Kate B CookTimothy R Hughes
Jan 22, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Xiaochen WangYun Yen
Feb 1, 2011·Wiley Interdisciplinary Reviews. RNA·Sandhya SandujaDan A Dixon
Jun 19, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Tao ZhangXiaodong Zhang
Jan 12, 2013·EMBO Molecular Medicine·Peter WendGustavo A Miranda-Carboni
Apr 27, 2013·Nucleic Acids Research·Stanislav BellaousovDavid H Mathews
May 21, 2013·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Jingjing Wu, Jian-Jun Wei
Mar 26, 2014·BMC Biology·Indrabahadur SinghGuillermo Barreto
May 2, 2014·Trends in Genetics : TIG·Andreas Wachter
Nov 5, 2014·Nature Communications·Je-Hyun YoonMyriam Gorospe
Nov 25, 2014·Cell·Agnieszka Rybak-WolfNikolaus Rajewsky
Mar 3, 2015·Biochemical and Biophysical Research Communications·Yuen GaoXiaodong Zhang
May 29, 2015·Nature Reviews. Molecular Cell Biology·Kaspar Burger, Monika Gullerova

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Citations

Nov 11, 2017·Medical Oncology·Jian GuoJiazeng Xia
Jul 18, 2018·Journal of Cellular Biochemistry·Asmaa Abd El GwadFathy Tash
Nov 2, 2018·International Journal of Molecular Sciences·Jeong-Min ParkTae-Hong Kang

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Methods Mentioned

BETA
PCR
immunoprecipitation
RIP-PCR
RIP
transfection
flow cytometry
surgical resection
ELISA

Software Mentioned

RNAstructure
RNAdraw
Augct
RNAhybrid
GraphPad Prism
CLC sequence viewer
Cell Quest

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