PMID: 6168579Jan 1, 1981Paper

The free and the beta 2-microglobulin-associated heavy chains of HLA-A, B alloantigens share the antigenic determinant recognized by the monoclonal antibody Q1/28

Immunogenetics
V QuarantaS Ferrone

Abstract

Serological and immunochemical assays have shown that the monoclonal antibody Q1/28 recognizes an antigenic determinant which is expressed on the heavy chain of subsets of HLA-A, B antigens and is distinct from those defining the serological polymorphism of this system. Association of the HLA-A, B heavy chain with beta 2-microglobulin is not required for expression of the antigenic determinant recognized by the monoclonal antibody Q1/28, since this antibody can immunoprecipitate a 45 000 m.w. component from radiolabeled lymphoid-cell glycoproteins immunodepleted with either an anti-human beta 2-microglobulin xenoantiserum or the MoAb W6/32 to framework determinants of HLA-A, B, C antigens. Furthermore, the MoAb Q1/28 can immunoprecipitate a 45 000 m.w. component from an NP40 lysate of radiolabeled Daudi cells, which lack the genetic information for beta 2-microglobulin. The determinant recognized by the MoAb Q1/28 is relatively resistant to denaturing treatments and does not appear to be carbohydrate in nature. The MoAb Q1/28 is the first example of an antibody which recognizes an antigenic determinant expressed on both the beta 2-microglobulin-associated and free HLA-A, B heavy chains.

References

Jan 1, 1977·Transplantation·S FerroneR A Reisfeld
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D LancetJ L Strominger
Dec 1, 1979·Cell·M S KrangelJ L Strominger
Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·F Hyafil, J L Strominger
May 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H L PloeghJ L Strominger
Jan 1, 1979·Journal of Immunological Methods·P G NataliR A Reisfeld
Mar 1, 1977·Somatic Cell Genetics·M L GefterM D Scharff
Feb 28, 1978·Biochemical and Biophysical Research Communications·P J Fraker, J C Speck
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Jun 1, 1960·Archives of Biochemistry and Biophysics·A NISONOFFD L WOERNLEY

❮ Previous
Next ❯

Citations

Apr 1, 1992·Gastroenterologia Japonica·K SakaguchiT Tsuji
Mar 26, 1982·Journal of Immunological Methods·L E Walker, R A Reisfeld
Jan 1, 1993·Journal of Reproductive Immunology·C J ThalerW P Faulk
Jul 1, 1989·Human Immunology·N CohenF C Grumet
Oct 24, 2002·Human Immunology·Giulio Lelio PalmisanoGiovanni Battista Ferrara
Mar 15, 2000·Human Immunology·M P PistilloG B Ferrara
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·W R ChurchR A Reisfeld
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·L E WalkerR A Reisfeld
Nov 1, 1982·The Journal of Experimental Medicine·V QuarantaR A Reisfeld
Sep 1, 1989·The Journal of Clinical Investigation·F Perosa, S Ferrone
Sep 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S A DesaiS Ferrone
Jan 1, 1993·International Journal of Clinical & Laboratory Research·S Demaria, Y Bushkin
Mar 7, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Aline MartayanPatrizio Giacomini
Mar 1, 1990·Cellular Immunology·Y BushkinJ M Le

❮ Previous
Next ❯