The frequency, growth kinetics, and osteogenic/adipogenic differentiation properties of canine bone marrow stromal cells.

In Vitro Cellular & Developmental Biology. Animal
Hiroaki KamishinaRoger M Clemmons

Abstract

Bone marrow stromal cells (BMSCs) have gained considerable attention as a potential source for cell transplantation therapies for a variety of diseases due to their accessibility, proliferative capacity, and multilineage differentiation properties. Canine BMSCs have been shown to contribute to regeneration of osseous tissues, but knowledge about their biology is currently limited. In the present study, we investigated the frequency of adult canine BMSCs in bone marrow, morphological features, growth kinetics, and osteogenic as well as adipogenic differentiation properties in vitro. Our data suggest that adult canine bone marrow contains approximately one BMSC in every 2.38 x 10(4) bone marrow mononucleated cells (0.0042 +/- 0.0019%, n = 5). Primary BMSC cultures consisted of morphologically heterogeneous adherent cell populations from which spindle-shaped cells grew and became the predominant cell type. Growth kinetics patterns were dependent on the initial cell seeding densities, resulting in the highest fold increase at lower cell density. In the presence of osteogenic and adipogenic inducers, primary BMSCs underwent morphological and phenotypic changes characteristic of osteogenic and adipogenic differentiation, respectively...Continue Reading

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Citations

Dec 3, 2009·The Journal of Veterinary Medical Science·Hiroaki KamishinaRoger M Clemmons
May 18, 2016·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·F Özdal-KurtS I Deliloğlu-Gürhan
Apr 2, 2014·Cell Biology International·Farzaneh KhademiJafar Ai

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Methods Mentioned

BETA
density gradient centrifugation
fluorescence-activated cell sorting
immunodepletion

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