The frequent detection of a treponeme in bovine digital dermatitis by immunocytochemistry and polymerase chain reaction

Veterinary Microbiology
I DemirkanM J Woodward

Abstract

A study was carried out to determine whether spirochaetes are frequently associated with digital dermatitis in United Kingdom (UK) dairy cattle. Histopathological examination of lesions using a silver stain showed a large number of unidentified spirochaete-like organisms present in digital dermatitis hoof skin tissue in all examined biopsies. Immunocytochemical staining demonstrated that spirochaetes in skin lesions were identified by polyclonal antisera to Borrelia burgdorferi, Treponema denticola and Treponema vincentii (again all biopsies were positively stained), whereas monoclonal antibodies to B. burgdorferi and any Treponema pallidum did not stain any organisms in all biopsies. A PCR of 16S rRNA, previously shown to be specific for a new treponeme, was employed and produced positive results from 82.4% of digital dermatitis tissues. It is concluded that this spirochaete (or related spirochaetes), which is similar to human oral treponemes, is frequently associated with, and may be responsible for, pathological changes in digital dermatitis.

References

Jan 18, 1992·The Veterinary Record·D H ReadM C Thurmond
May 21, 1988·The Veterinary Record·R W Blowey, M W Sharp
Sep 26, 1987·The Veterinary Record·R W Blowey
Nov 22, 1997·FEMS Microbiology Letters·R J Collighan, M J Woodward

❮ Previous
Next ❯

Citations

Nov 22, 2002·Research in Veterinary Science·R D MurrayS D Carter
Jul 5, 2013·Animal : an International Journal of Animal Bioscience·M A PalmerN E O'Connell
Sep 13, 2002·Australian Veterinary Journal·T ShibaharaK Kadota
Jan 16, 2009·Journal of Clinical Microbiology·Nicholas J EvansStuart D Carter
Aug 22, 2000·American Journal of Veterinary Research·C C BrownK L Jacobsen
Nov 1, 2011·Preventive Veterinary Medicine·B H NielsenJ Kaler
Dec 17, 2008·Veterinary Microbiology·Nicholas J EvansStuart D Carter
Feb 5, 2008·Veterinary Microbiology·Nicholas J EvansStuart D Carter
Nov 21, 2007·Veterinary Microbiology·Sebastian SchlaferAnnette Moter
Apr 12, 2016·The Veterinary Journal·N J EvansS D Carter
May 29, 2000·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·A M Rashmir-RavenM Akin
Mar 20, 2015·Journal of Clinical Microbiology·Ligia Valéria NascimentoRüdiger Daniel Ollhoff
Dec 22, 1999·The Veterinary Record·I DemirkanM J Woodward
Mar 19, 2005·International Journal of Systematic and Evolutionary Microbiology·C WyssA Luginbühl
Jan 1, 2013·F1000Research·Marianne J MiddelveenRaphael B Stricker

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.