The fruit ripening-related gene FaAAT2 encodes an acyl transferase involved in strawberry aroma biogenesis

Journal of Experimental Botany
Guadalupe Cumplido-LasoR Blanco-Portales

Abstract

Short-chain esters contribute to the blend of volatiles that define the strawberry aroma. The last step in their biosynthesis involves an alcohol acyltransferase that catalyses the esterification of an acyl moiety of acyl-CoA with an alcohol. This study identified a novel strawberry alcohol acyltransferase gene (FaAAT2) whose expression pattern during fruit receptacle growth and ripening is in accordance with the production of esters throughout strawberry fruit ripening. The full-length FaAAT2 cDNA was cloned and expressed in Escherichia coli and its activity was analysed with acyl-CoA and alcohol substrates. The semi-purified FaAAT2 enzyme had activity with C1-C8 straight-chain alcohols and aromatic alcohols in the presence of acetyl-CoA. Cinnamyl alcohol was the most efficient acyl acceptor. When FaAAT2 expression was transiently downregulated in the fruit receptacle by agroinfiltration, the volatile ester production was significantly reduced in strawberry fruit. The results suggest that FaAAT2 plays a significant role in the production of esters that contribute to the final strawberry fruit flavour.

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Citations

Dec 13, 2012·Plant & Cell Physiology·Enriqueta Moyano-CañeteRosario Blanco-Portales
Nov 28, 2013·Journal of Experimental Botany·Laura Medina-PucheRosario Blanco-Portales
Sep 11, 2013·Biotechnology Journal·Irene Aragüez, Victoriano Valpuesta
Mar 26, 2014·The Plant Journal : for Cell and Molecular Biology·Edwige J F SouleyreRoss G Atkinson
Aug 11, 2015·Plant Science : an International Journal of Experimental Plant Biology·Francisco J Molina-HidalgoRosario Blanco-Portales
Jul 1, 2015·Frontiers in Plant Science·Michela Guidarelli, Elena Baraldi
Sep 8, 2016·Horticulture Research·Kevin M Folta, Harry J Klee
Oct 21, 2016·Functional & Integrative Genomics·Laura Medina-PucheAntonio Rodríguez-Franco
May 27, 2017·Journal of Proteomics·Boon Chin TanSu-Ee Lau
Apr 1, 2018·Journal of the Science of Food and Agriculture·Jia-Wei YanLi Li
Jan 10, 2020·Proceedings of the National Academy of Sciences of the United States of America·Pachara SattayawatPatrik R Jones
Jan 20, 2019·Applied Microbiology and Biotechnology·Hyeon Ji NohYu-Sin Jang
Oct 6, 2017·Journal of Experimental Botany·Francisco Javier Molina-HidalgoEnriqueta Moyano
Dec 29, 2019·BMC Plant Biology·Laura Medina-PucheRosario Blanco-Portales
Feb 18, 2021·The Plant Journal : for Cell and Molecular Biology·Xiangmei CaoBo Zhang
May 2, 2015·Plant Physiology·Laura Medina-PucheJuan Muñoz-Blanco
Jun 22, 2021·Critical Reviews in Biotechnology·Linlin WangXue Qiao
Apr 19, 2019·Journal of Agricultural and Food Chemistry·Xiangmei CaoBo Zhang

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