The FTF gene family regulates virulence and expression of SIX effectors in Fusarium oxysporum

Molecular Plant Pathology
Jonathan Niño-SánchezJosé María Díaz Mínguez

Abstract

The FTF (Fusarium transcription factor) gene family comprises a single copy gene, FTF2, which is present in all the filamentous ascomycetes analysed, and several copies of a close relative, FTF1, which is exclusive to Fusarium oxysporum. An RNA-mediated gene silencing system was developed to target mRNA produced by all the FTF genes, and tested in two formae speciales: F. oxysporum f. sp. phaseoli (whose host is common bean) and F. oxysporum f. sp. lycopersici (whose host is tomato). Quantification of the mRNA levels showed knockdown of FTF1 and FTF2 in randomly isolated transformants of both formae speciales. The attenuation of FTF expression resulted in a marked reduction in virulence, a reduced expression of several SIX (Secreted In Xylem) genes, the best studied family of effectors in F. oxysporum, and lower levels of SGE1 (Six Gene Expression 1) mRNA, the presumptive regulator of SIX expression. Moreover, the knockdown mutants showed a pattern of colonization of the host plant similar to that displayed by strains devoid of FTF1 copies (weakly virulent strains). Gene knockout of FTF2 also resulted in a reduction in virulence, but to a lesser extent. These results demonstrate the role of the FTF gene expansion, mostly the FT...Continue Reading

References

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Citations

Mar 17, 2018·Molecular Plant-microbe Interactions : MPMI·Amjad M HusainiSouliha R Cambay
Jun 25, 2017·Annual Review of Phytopathology·H Charlotte van der Does, Martijn Rep
May 7, 2020·Pathogens·Sephra N Rampersad
Jul 22, 2020·Phytopathology·He YangLi-Jun Ma
Apr 16, 2020·Frontiers in Bioengineering and Biotechnology·Yu-Ke CenYu-Guo Zheng
Jan 10, 2020·BMC Genomics·Laura Hernandez-EscribanoSanushka Naidoo
Jan 11, 2020·Frontiers in Microbiology·Andrew TaylorJohn P Clarkson
May 11, 2021·Molecular Plant Pathology·Qussai ZuriegatYingzi Yun
May 12, 2021·Molecular Plant Pathology·Evan JohnKar-Chun Tan
May 11, 2021·Frontiers in Plant Science·Pooja JangirRupam Kapoor

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Datasets Mentioned

BETA
JN167165

Methods Mentioned

BETA
PCR
PCRs

Software Mentioned

GBLOCKS
Geneious
ImageJ
lustal W
blast
PhyML
MUSCLE

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