The ftsZ gene as a tool for detection of Mycoplasma fermentans

Molecular and Cellular Probes
R OfirY Weinstein

Abstract

Mycoplasma fermentans was reported as a common contaminant of cell cultures, and was shown to either induce or suppress several immunological functions. A strain of M. fermentans was recently isolated from a mouse T-lymphoma cell line, which differs from other M. fermentans strains by its growth characteristics and was designated (in the authors' records) as strain 609. Using the differential display technique (DD), a differentially expressed gene that was identified as the M. fermentans 609 ftsZ gene was isolated. Comparison of the nucleotide sequence of the M. fermentans 609 ftsZ gene to other ftsZ genes showed a 98% homology with Mycoplasma fermentans strain K7 and approximately 50% homology with Mycoplasma pulmonis and Mycoplasma genitalium. Comparison of the putative amino acid sequences of the FtsZ proteins showed similar homology. A polymerase chain reaction (PCR) assay to detect the presence of this ftsZ gene was established; it is a fast and convenient assay to detect infection of cells by the M. fermentans species. This work demonstrates that: (i) DD can be used as a useful technique to identify and isolate mycoplasmal genes from infected cells; and (ii) the ftsZ gene can be a useful marker to distinguish between diff...Continue Reading

References

Aug 1, 1992·Leukemia Research·R OfirY Weinstein
Sep 17, 1992·Nature·P de BoerL Rothfield
Jan 1, 1990·Leukemia Research·E Aflalo, Y Weinstein
Dec 1, 1994·Molecular and Cellular Probes·S Razin
Jan 1, 1994·Annual Review of Microbiology·A Blanchard, L Montagnier
Feb 10, 1995·Cell·H P Erickson
Mar 1, 1994·Applied and Environmental Microbiology·O Dussurget, D Roulland-Dussoix
Aug 1, 1993·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·A BlanchardG H Cassell
May 1, 1997·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·R OfirY Weinstein

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Citations

Sep 26, 2007·Biologicals : Journal of the International Association of Biological Standardization·Baharak AfsharRoger J Miles
Aug 17, 1999·Biochemical and Biophysical Research Communications·A V KukekovaS N Borchsenius
Jun 11, 2002·Biochemical and Biophysical Research Communications·K T MomynalievV M Govorun

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