PMID: 7028483Sep 1, 1981Paper

The function of ribosomal protein S21 in protein synthesis

European Journal of Biochemistry
J van Duin, R Wijnands

Abstract

The function of ribosomal protein S21 in protein synthesis has been examined by (a) inactivation of S21 in situ with specific antibodies and (b) the use of 30-S subunits reconstituted in the absence of S21. The results from the two approaches are consistent, 30-S subunits treated with anti-S21 or lacking S21 are still active in the translation of poly(U) or poly(A, G, U). They are also functional in fMet-tRNA binding when directed by poly(A, G, U) or the AUG triplet. They are not active in the translation of MS2 RNA or Escherichia coli mRNA. The defect of S21-deficient 30-S ribosomes can be traced back to their inability to bind MS2 RNA at the initiation step of protein synthesis. Addition of S21 to S21-deprived subunits restores the MS2-RNA-dependent initiation complex formation.

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Citations

Jan 1, 1982·Progress in Biophysics and Molecular Biology·A Liljas
Oct 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Andrei KorostelevHarry F Noller
Sep 25, 1990·Nucleic Acids Research·C D Prescott, H U Göringer
May 7, 2014·Proceedings of the National Academy of Sciences of the United States of America·Prem S KaushalRajendra K Agrawal
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Sep 10, 2014·Nature Structural & Molecular Biology·Neha Gupta, Gloria M Culver
Jul 17, 2019·Proceedings of the National Academy of Sciences of the United States of America·Jacob R WaldbauerLichun Zhang
Feb 16, 2019·Nature Communications·Carolina M MizunoMart Krupovic
Mar 18, 2020·Nucleic Acids Research·Sabrina SummerAlexandre Smirnov
Feb 20, 2020·MSystems·Asier Zaragoza-SolasMario López-Pérez
Feb 14, 2020·Nature·Basem Al-ShayebJillian F Banfield
Jul 19, 2020·Molecular Biology and Evolution·Daria D NikolaevaSofya K Garushyants
Jun 20, 1988·Journal of Molecular Biology·S SternH F Noller
Jul 15, 2018·Microbiology Spectrum·Heather J Beck, Isabella Moll

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