The function of the sodium pump during differentiation of amphibian embryonic neurones

The Journal of Physiology
E A Messenger, A E Warner

Abstract

1. A method has been developed for studying the differentiation in tissue culture of ectoderm and mesoderm derivatives, dissected from amphibian embryos which have just completed neurulation. 2. Neurones, striated muscle cells and pigment cells, together with other unidentifiable cell types, differentiated as a monolayer with approximately the same time course as in the whole embryo. The proportion of different cell types in the cultures was measured quantitatively by cell counting. 3. Treatment of embryos during neurulation with the cardiac glycoside strophanthidin reduced the number of neurones which subsequently differentiated in culture. Other cell types were not affected. 4. The relationship between inhibition of neural differentiation and strophanthidin concentration was sigmoid, with maximum inhibition at 10(-5) M-strophanthidin and the mid-point at 5 X 10(-7) M-strophanthidin. 35% of neurones differentiating in culture were not affected by glycoside treatment. 5. The glycoside hexahydroscillaren A had no effect on neural differentiation. 6. Increasing extracellular potassium to 100 nM during strophanthidin treatment completely protected differentiating neurones from the inhibitory effect of strophanthidin. 7. Treatment ...Continue Reading

Citations

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