The function role of miR-181a in chemosensitivity to adriamycin by targeting Bcl-2 in low-invasive breast cancer cells

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Ying ZhuJinhai Tang

Abstract

miR-181a is involved in immunity, metabolism, tumor suppression or carcinogenesis reported by many other studies. However, its role in the development of chemosensitivity to adriamycin in low-invasive breast cancer cells remains unclear. The aim of this study is to define the function role of miR-181a in promoting the efficacy of adriamycin-based neoadjuvant chemotherapy. Cell survival analysis was detected by Cell Counting Kit-8 assay. Apoptotic cells were quantitatively detected using FITC Annexin V apoptosis Detection Kit I. Bcl-2 protein expression was measured by western blot. Luciferase reporter vector with the putative BCL-2 3' untranslated region (3'UTR) was constructed to explore whether BCL-2 was a direct target gene of miR-181a. Real-time PCR was performed to test the expression of miR-181a and Bcl-2 in the selected breast cancer tissue samples. The down-regulation of miR-181a decreased adriamycin-induced apoptosis in MCF-7 cells. Transfected with miR-181a mimic in cells resulted in the decreased expression of Bcl-2. The alteration of miR-181a expression did not significantly affect the chemosensitivity to adriamycin in MCF-7 and MCF-7/ADR cells with genetic knockout of Bcl-2. miR-181a may suppress Bcl-2 expression b...Continue Reading

Citations

Mar 25, 2015·Journal of Cellular Biochemistry·Eduardo TormoPilar Eroles
Oct 18, 2016·Frontiers in Genetics·Shivani SharmaRitu Kulshreshtha
Dec 3, 2014·Biochemical and Biophysical Research Communications·Jing WuXia He
May 6, 2019·International Journal of Molecular Sciences·Natalia García-SanchaJavier Cañueto
Jun 4, 2019·Journal of Cellular Biochemistry·Wei ZhangFeng Ren
Jul 20, 2019·IUBMB Life·Hamid NajminejadMohammad Hasan Sheikhha
Dec 28, 2019·Journal of Cellular and Molecular Medicine·Juntao KeXiaoping Miao
Aug 16, 2016·Oncotarget·Xiaomeng HuangGuido Marcucci
Feb 13, 2019·Clinical Epigenetics·Wengong SiWeimin Fan
Sep 17, 2020·Pharmaceuticals·Shabirul Haque, Sarah R Vaiselbuh
Aug 9, 2020·OncoTargets and Therapy·Tonghua DuBin Liu
Apr 30, 2017·RNA·Shivani SharmaRitu Kulshreshtha
Mar 21, 2020·Frontiers in Oncology·Ying XiangShuai-Shuai Niu
Nov 11, 2019·Molecular Aspects of Medicine·Cornelia BraicuIoana Berindan-Neagoe
Jan 12, 2021·Biochemical Pharmacology·Shilpi SinghSuaib Luqman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Breast Cancer: Chemo-Resistance

Some cancers are difficult to treat and aggressive including the "triple-negative" breast cancer. This type of cancer is chemoresistant even before chemotherapy begins. Here are the latest discoveries chemo-resistance in breast cancer.

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.

Carcinoma, Lobular

Lobular carcinoma is an invasive type of breast cancer in which lobules, areas of the breast that produce milk, undergo malignant transformation. Here is the latest research.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Breast Cancer: Risk Factors

Breast cancer is a multifactorial disease that is influenced by both environmental and genetic factors. Discover the latest research on the environmental and genetic risk factors for breast cancer here.

B-Cell Leukemia (Keystone)

B-cell leukemia includes various types of lymphoid leukemia that affect B cells. Here is the latest research on B-cell leukemia.