The functional characterization of normal and neoplastic human enterochromaffin cells

The Journal of Clinical Endocrinology and Metabolism
Irvin M ModlinManish C Champaneria

Abstract

Neuroendocrine regulation of small intestinal (SI) function is poorly understood because pure neuroendocrine cells are unavailable, whereas the biological basis of SI carcinoid tumors is unknown because neoplastic human enterochromaffin (EC) cells are unavailable. The objective of this study was to define the secretory regulation and transcriptome of naive and neoplastic SI neuroendocrine cells. EC cells from human ilea were isolated and purified, and a malignant EC cell carcinoid cell line (KRJ-I) was characterized. Human ilea from right hemicolectomies were pronase/collagenase digested and Nycodenz gradient centrifuged, and EC cells were fluorescence-activated cell sorting (FACS) sorted after acridine orange labeling. Enrichment was defined by immunostaining, gene expression, serotonin (5-HT) content, and real-time RT-PCR. Naive FACS-sorted EC and KRJ-I cells were cultured, and 5-HT secretion was measured after stimulation with forskolin, isoproterenol, acetylcholine, gamma-aminobutyric acid A (GABA(A)), pituitary adenylate cyclase-activating polypeptide (PACAP)-38, and gastrin. Normal and neoplastic EC cell transcriptomes were acquired by Affymetrix profiling (U133A). FACS produced 100 +/- 0.3% (chromogranin A staining) and ...Continue Reading

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Citations

May 19, 2011·Cancer Immunology, Immunotherapy : CII·Chiara TieppoFabio Farinati
Jan 29, 2011·Langenbeck's Archives of Surgery·Simon SchimmackIrvin M Modlin
Sep 27, 2006·Pituitary·Eva Hofsli
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Jan 20, 2018·BioMed Research International·Krystallenia I AlexandrakiGregory A Kaltsas

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